| Back to Multiple platform build/check report for BioC 3.14 |
|
This page was generated on 2022-04-13 12:07:17 -0400 (Wed, 13 Apr 2022).
| Hostname | OS | Arch (*) | R version | Installed pkgs |
|---|---|---|---|---|
| nebbiolo2 | Linux (Ubuntu 20.04.4 LTS) | x86_64 | 4.1.3 (2022-03-10) -- "One Push-Up" | 4324 |
| tokay2 | Windows Server 2012 R2 Standard | x64 | 4.1.3 (2022-03-10) -- "One Push-Up" | 4077 |
| machv2 | macOS 10.14.6 Mojave | x86_64 | 4.1.3 (2022-03-10) -- "One Push-Up" | 4137 |
| Click on any hostname to see more info about the system (e.g. compilers) (*) as reported by 'uname -p', except on Windows and Mac OS X | ||||
|
To the developers/maintainers of the sangeranalyseR package: - Please allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/sangeranalyseR.git to reflect on this report. See How and When does the builder pull? When will my changes propagate? for more information. - Make sure to use the following settings in order to reproduce any error or warning you see on this page. |
| Package 1691/2083 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | ||||||||
| sangeranalyseR 1.4.0 (landing page) Kuan-Hao Chao
| nebbiolo2 | Linux (Ubuntu 20.04.4 LTS) / x86_64 | OK | OK | WARNINGS |  | ||||||||
| tokay2 | Windows Server 2012 R2 Standard / x64 | OK | OK | WARNINGS | OK | | ||||||||
| machv2 | macOS 10.14.6 Mojave / x86_64 | OK | OK | WARNINGS | OK | | ||||||||
| Package: sangeranalyseR |
| Version: 1.4.0 |
| Command: C:\Users\biocbuild\bbs-3.14-bioc\R\bin\R.exe CMD check --force-multiarch --install=check:sangeranalyseR.install-out.txt --library=C:\Users\biocbuild\bbs-3.14-bioc\R\library --no-vignettes --timings sangeranalyseR_1.4.0.tar.gz |
| StartedAt: 2022-04-13 02:14:32 -0400 (Wed, 13 Apr 2022) |
| EndedAt: 2022-04-13 02:23:53 -0400 (Wed, 13 Apr 2022) |
| EllapsedTime: 560.8 seconds |
| RetCode: 0 |
| Status: WARNINGS |
| CheckDir: sangeranalyseR.Rcheck |
| Warnings: 7 |
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###
### Running command:
###
### C:\Users\biocbuild\bbs-3.14-bioc\R\bin\R.exe CMD check --force-multiarch --install=check:sangeranalyseR.install-out.txt --library=C:\Users\biocbuild\bbs-3.14-bioc\R\library --no-vignettes --timings sangeranalyseR_1.4.0.tar.gz
###
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* using log directory 'C:/Users/biocbuild/bbs-3.14-bioc/meat/sangeranalyseR.Rcheck'
* using R version 4.1.3 (2022-03-10)
* using platform: x86_64-w64-mingw32 (64-bit)
* using session charset: ISO8859-1
* using option '--no-vignettes'
* checking for file 'sangeranalyseR/DESCRIPTION' ... OK
* checking extension type ... Package
* this is package 'sangeranalyseR' version '1.4.0'
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... NOTE
Depends: includes the non-default packages:
'stringr', 'ape', 'Biostrings', 'DECIPHER', 'parallel', 'reshape2',
'phangorn', 'sangerseqR', 'gridExtra', 'shiny', 'shinydashboard',
'shinyjs', 'data.table', 'plotly', 'DT', 'zeallot', 'excelR',
'shinycssloaders', 'ggdendro', 'shinyWidgets', 'openxlsx', 'tools',
'rmarkdown', 'knitr', 'seqinr', 'BiocStyle', 'logger'
Adding so many packages to the search path is excessive and importing
selectively is preferable.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... NOTE
Found the following hidden files and directories:
.travis.yml
docs/build/html/.buildinfo
These were most likely included in error. See section 'Package
structure' in the 'Writing R Extensions' manual.
* checking for portable file names ... WARNING
Found the following file with a non-portable file name:
docs/wallpaperflare.com_wallpaper (1).jpg
These are not fully portable file names.
See section 'Package structure' in the 'Writing R Extensions' manual.
Found the following non-portable file paths:
sangeranalyseR/docs/build/html/_downloads/081987116457968e1b81ae1616525c7d/Sanger_contigs_unalignment.fa
sangeranalyseR/docs/build/html/_downloads/13b875bc704f73b8bd67dc07d86fc474/Sanger_contigs_alignment.fa
sangeranalyseR/docs/build/html/_downloads/144d09562ae5b2c7dc079e4bc2b33fb7/Sanger_contigs_alignment.fa
sangeranalyseR/docs/build/html/_downloads/165af37e8893cfdfa4dba91c44cd791b/Sanger_contigs_unalignment.fa
sangeranalyseR/docs/build/html/_downloads/345ac400befc4f176c75732770ec3fcb/Achl_RBNII384-13_contig.fa
sangeranalyseR/docs/build/html/_downloads/438c8bd47d93d92c65b35b613ef14739/Sanger_all_trimmed_reads.fa
sangeranalyseR/docs/build/html/_downloads/6211ea1e7e3ccc1fcc9b74b78224eab6/Achl_ACHLO006-09_reads_unalignment.fa
sangeranalyseR/docs/build/html/_downloads/67bcad64cca804ec2e14cc42d016c08f/Achl_ACHLO006-09_reads_alignment.fa
sangeranalyseR/docs/build/html/_downloads/81a7338101f110748cb72056d9975225/Sanger_all_trimmed_reads.fa
sangeranalyseR/docs/build/html/_downloads/95ef9378c1407481d69c3bf882a580bb/Achl_RBNII384-13_reads_unalignment.fa
sangeranalyseR/docs/build/html/_downloads/a664bed9cc5d32924dc2ce60f8c60554/Sanger_all_trimmed_reads.fa
sangeranalyseR/docs/build/html/_downloads/b608796fffaaa7335a7341cffc9a9db7/Achl_ACHLO006-09_contig.fa
sangeranalyseR/docs/build/html/_downloads/b6b18aa3e34c6ae0be86c3940972dd38/Sanger_contigs_alignment.fa
sangeranalyseR/docs/build/html/_downloads/eeb554b575ee5b29ef733dafbf876c36/Achl_RBNII384-13_reads_alignment.fa
sangeranalyseR/docs/build/html/_downloads/fef71e7b2e1e49c01df301435e972047/Sanger_contigs_unalignment.fa
Tarballs are only required to store paths of up to 100 bytes and cannot
store those of more than 256 bytes, with restrictions including to 100
bytes for the final component.
See section 'Package structure' in the 'Writing R Extensions' manual.
* checking whether package 'sangeranalyseR' can be installed ... OK
* checking installed package size ... NOTE
installed size is 9.0Mb
sub-directories of 1Mb or more:
data 2.2Mb
extdata 5.5Mb
* checking package directory ... OK
* checking 'build' directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... NOTE
File
LICENSE
is not mentioned in the DESCRIPTION file.
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... WARNING
Found the following files with non-ASCII characters:
ClassSangerAlignment.R
ClassSangerContig.R
ClassSangerRead.R
Portable packages must use only ASCII characters in their R code,
except perhaps in comments.
Use \uxxxx escapes for other characters.
* checking R files for syntax errors ... OK
* loading checks for arch 'i386'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* loading checks for arch 'x64'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... NOTE
Packages in Depends field not imported from:
'BiocStyle' 'knitr' 'phangorn' 'reshape2' 'zeallot'
These packages need to be imported from (in the NAMESPACE file)
for when this namespace is loaded but not attached.
package 'methods' is used but not declared
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
QualityBasePlotly: no visible global function definition for '%>%'
SangerAlignment: no visible global function definition for 'new'
SangerAlignmentServer: no visible global function definition for '%>%'
SangerAlignmentServer: no visible binding for '<<-' assignment to
'NEW_SANGER_ALIGNED_CONSENSUS_READ_SET'
SangerAlignmentServer: no visible global function definition for
'colorRamp'
SangerContig: no visible global function definition for 'new'
SangerContigServer: no visible global function definition for '%>%'
SangerContigServer: no visible binding for '<<-' assignment to
'NEW_SANGER_CONTIG'
SangerContigServer: no visible global function definition for
'colorRamp'
SangerRead: no visible global function definition for 'new'
SetCharStyleList: no visible global function definition for '%>%'
alignContigs: no visible global function definition for 'as.phylo'
alignContigs: no visible global function definition for 'rtree'
checkAb1FastaCsv: no visible global function definition for 'read.csv'
checkTargetFastaName: no visible global function definition for
'isEmpty'
chromatogram_overwrite: no visible global function definition for 'rgb'
chromatogram_overwrite: no visible global function definition for 'par'
chromatogram_overwrite: no visible global function definition for
'quantile'
chromatogram_overwrite: no visible global function definition for 'IQR'
chromatogram_overwrite: no visible global function definition for 'pdf'
chromatogram_overwrite: no visible global function definition for
'rect'
chromatogram_overwrite: no visible global function definition for
'lines'
chromatogram_overwrite: no visible global function definition for
'mtext'
chromatogram_overwrite: no visible global function definition for
'axis'
chromatogram_overwrite: no visible global function definition for
'dev.off'
primarySeqDisplay: no visible global function definition for 'rgb'
primarySeqTrimmedDisplay: no visible global function definition for
'rgb'
secondSeqTrimmedDisplay: no visible global function definition for
'rgb'
secondarySeqDisplay: no visible global function definition for 'rgb'
initialize,ChromatogramParam: no visible global function definition for
'callNextMethod'
initialize,ObjectResults: no visible global function definition for
'callNextMethod'
initialize,QualityReport: no visible global function definition for
'callNextMethod'
initialize,SangerAlignment: no visible global function definition for
'str_split'
initialize,SangerAlignment : <anonymous>: no visible global function
definition for 'new'
initialize,SangerAlignment: no visible global function definition for
'read.csv'
initialize,SangerAlignment: no visible global function definition for
'new'
initialize,SangerAlignment: no visible global function definition for
'callNextMethod'
initialize,SangerContig : <anonymous>: no visible global function
definition for 'new'
initialize,SangerContig: no visible global function definition for
'read.csv'
initialize,SangerContig: no visible global function definition for
'new'
initialize,SangerContig: no visible global function definition for
'callNextMethod'
initialize,SangerRead: no visible global function definition for 'new'
initialize,SangerRead: no visible global function definition for
'AAString'
initialize,SangerRead: no visible global function definition for
'callNextMethod'
launchAppSA,SangerAlignment: no visible global function definition for
'shinyOptions'
launchAppSA,SangerAlignment: no visible global function definition for
'shinyApp'
launchAppSC,SangerContig: no visible global function definition for
'shinyOptions'
launchAppSC,SangerContig: no visible global function definition for
'shinyApp'
Undefined global functions or variables:
%>% AAString IQR as.phylo axis callNextMethod colorRamp dev.off
isEmpty lines mtext new par pdf quantile read.csv rect rgb rtree
shinyApp shinyOptions str_split
Consider adding
importFrom("grDevices", "colorRamp", "dev.off", "pdf", "rgb")
importFrom("graphics", "axis", "lines", "mtext", "par", "rect")
importFrom("methods", "callNextMethod", "new")
importFrom("stats", "IQR", "quantile")
importFrom("utils", "read.csv")
to your NAMESPACE file (and ensure that your DESCRIPTION Imports field
contains 'methods').
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... WARNING
Undocumented code objects:
'chromatogram_overwrite'
All user-level objects in a package should have documentation entries.
See chapter 'Writing R documentation files' in the 'Writing R
Extensions' manual.
* checking for code/documentation mismatches ... WARNING
S4 class codoc mismatches from documentation object 'ObjectResults-class':
Slots for class 'ObjectResults'
Code: creationResult errorMessages errorTypes printLevel
readResultTable warningMessages warningTypes
Docs: printLevel
* checking Rd \usage sections ... WARNING
Undocumented arguments in documentation object 'SangerAlignment'
'printLevel' 'processMethod'
Documented arguments not in \usage in documentation object 'SangerAlignment':
'minFractionCallSA' 'maxFractionLostSA'
Undocumented arguments in documentation object 'SangerContig'
'printLevel' 'processMethod'
Undocumented arguments in documentation object 'SangerRead'
'printLevel'
Functions with \usage entries need to have the appropriate \alias
entries, and all their arguments documented.
The \usage entries must correspond to syntactically valid R code.
See chapter 'Writing R documentation files' in the 'Writing R
Extensions' manual.
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of 'data' directory ... OK
* checking data for non-ASCII characters ... WARNING
Failed with error: 'package 'phangorn' could not be loaded'
Error loading dataset 'qualityReportData':
Error in .requirePackage(package) :
unable to find required package 'sangeranalyseR'
Failed with error: 'package 'phangorn' could not be loaded'
Error loading dataset 'sangerAlignmentData':
Error in .requirePackage(package) :
unable to find required package 'sangeranalyseR'
Failed with error: 'package 'phangorn' could not be loaded'
Error loading dataset 'sangerContigData':
Error in .requirePackage(package) :
unable to find required package 'sangeranalyseR'
Failed with error: 'package 'phangorn' could not be loaded'
Error loading dataset 'sangerReadFData':
Error in .requirePackage(package) :
unable to find required package 'sangeranalyseR'
The dataset(s) may use package(s) not declared in the DESCRIPTION file.
* checking data for ASCII and uncompressed saves ... WARNING
Note: significantly better compression could be obtained
by using R CMD build --resave-data
old_size new_size compress
sangerAlignmentData.RData 1.5Mb 700Kb xz
sangerContigData.RData 451Kb 204Kb xz
sangerReadFData.RData 269Kb 119Kb xz
* checking line endings in Makefiles ... OK
* checking for GNU extensions in Makefiles ... OK
* checking include directives in Makefiles ... OK
* checking files in 'vignettes' ... OK
* checking examples ...
** running examples for arch 'i386' ... OK
Examples with CPU (user + system) or elapsed time > 5s
user system elapsed
SangerAlignment-class 53.56 0.83 54.94
SangerAlignment 12.67 0.03 14.56
SangerContig-class 10.55 0.08 10.64
** running examples for arch 'x64' ... OK
Examples with CPU (user + system) or elapsed time > 5s
user system elapsed
SangerAlignment-class 52.09 0.40 52.83
SangerAlignment 11.36 0.06 11.42
SangerContig-class 11.06 0.08 11.14
* checking for unstated dependencies in 'tests' ... OK
* checking tests ...
** running tests for arch 'i386' ...
Running 'testthat.R'
OK
** running tests for arch 'x64' ...
Running 'testthat.R'
OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in 'inst/doc' ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE
Status: 7 WARNINGs, 6 NOTEs
See
'C:/Users/biocbuild/bbs-3.14-bioc/meat/sangeranalyseR.Rcheck/00check.log'
for details.
sangeranalyseR.Rcheck/00install.out
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###
### Running command:
###
### C:\cygwin\bin\curl.exe -O http://155.52.207.166/BBS/3.14/bioc/src/contrib/sangeranalyseR_1.4.0.tar.gz && rm -rf sangeranalyseR.buildbin-libdir && mkdir sangeranalyseR.buildbin-libdir && C:\Users\biocbuild\bbs-3.14-bioc\R\bin\R.exe CMD INSTALL --merge-multiarch --build --library=sangeranalyseR.buildbin-libdir sangeranalyseR_1.4.0.tar.gz && C:\Users\biocbuild\bbs-3.14-bioc\R\bin\R.exe CMD INSTALL sangeranalyseR_1.4.0.zip && rm sangeranalyseR_1.4.0.tar.gz sangeranalyseR_1.4.0.zip
###
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% Total % Received % Xferd Average Speed Time Time Time Current
Dload Upload Total Spent Left Speed
0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0
1 41.4M 1 470k 0 0 701k 0 0:01:00 --:--:-- 0:01:00 701k
4 41.4M 4 1986k 0 0 1189k 0 0:00:35 0:00:01 0:00:34 1189k
10 41.4M 10 4367k 0 0 1638k 0 0:00:25 0:00:02 0:00:23 1638k
17 41.4M 17 7596k 0 0 2082k 0 0:00:20 0:00:03 0:00:17 2081k
27 41.4M 27 11.5M 0 0 2545k 0 0:00:16 0:00:04 0:00:12 2545k
39 41.4M 39 16.5M 0 0 3005k 0 0:00:14 0:00:05 0:00:09 3316k
54 41.4M 54 22.4M 0 0 3465k 0 0:00:12 0:00:06 0:00:06 4229k
70 41.4M 70 29.2M 0 0 3925k 0 0:00:10 0:00:07 0:00:03 5150k
80 41.4M 80 33.4M 0 0 3957k 0 0:00:10 0:00:08 0:00:02 5326k
92 41.4M 92 38.4M 0 0 4076k 0 0:00:10 0:00:09 0:00:01 5498k
100 41.4M 100 41.4M 0 0 4170k 0 0:00:10 0:00:10 --:--:-- 5622k
install for i386
* installing *source* package 'sangeranalyseR' ...
** using staged installation
** R
** data
** inst
** byte-compile and prepare package for lazy loading
Note: wrong number of arguments to '=='
** help
*** installing help indices
converting help for package 'sangeranalyseR'
finding HTML links ... done
ChromatogramParam-class html
MakeBaseCalls-methods html
ObjectResults-class html
QualityReport-class-qualityBasePlot html
QualityReport-class-updateQualityParam
html
QualityReport-class html
SangerAlignment-class-generateReportSA
html
SangerAlignment-class-launchAppSA html
SangerAlignment-class-updateQualityParam
html
SangerAlignment-class-writeFastaSA html
SangerAlignment-class html
SangerAlignment html
SangerContig-class-generateReportSC html
SangerContig-class-launchAppSC html
SangerContig-class-readTable html
SangerContig-class-updateQualityParam html
SangerContig-class-writeFastaSC html
SangerContig-class html
SangerContig html
SangerRead-class-MakeBaseCalls html
SangerRead-class-generateReportSR html
SangerRead-class-qualityBasePlot html
SangerRead-class-readTable html
SangerRead-class-updateQualityParam html
SangerRead-class-writeFastaSR html
SangerRead-class html
SangerRead html
generateReport html
generateReportSA-methods html
generateReportSC-methods html
generateReportSR-methods html
launchApp html
launchAppSA-methods html
launchAppSC-methods html
qualityBasePlot-methods html
qualityReportData html
readTable-methods html
sangerAlignmentData html
sangerContigData html
sangerReadFData html
sangeranalyseR html
updateQualityParam-methods html
writeFasta html
writeFastaSA-methods html
writeFastaSC-methods html
writeFastaSR-methods html
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
install for x64
* installing *source* package 'sangeranalyseR' ...
** testing if installed package can be loaded
* MD5 sums
packaged installation of 'sangeranalyseR' as sangeranalyseR_1.4.0.zip
* DONE (sangeranalyseR)
* installing to library 'C:/Users/biocbuild/bbs-3.14-bioc/R/library'
package 'sangeranalyseR' successfully unpacked and MD5 sums checked
|
sangeranalyseR.Rcheck/tests_i386/testthat.Rout
R version 4.1.3 (2022-03-10) -- "One Push-Up"
Copyright (C) 2022 The R Foundation for Statistical Computing
Platform: i386-w64-mingw32/i386 (32-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(sangeranalyseR)
Loading required package: stringr
Loading required package: ape
Loading required package: Biostrings
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
tapply, union, unique, unsplit, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Attaching package: 'IRanges'
The following object is masked from 'package:grDevices':
windows
Loading required package: XVector
Loading required package: GenomeInfoDb
Attaching package: 'Biostrings'
The following object is masked from 'package:ape':
complement
The following object is masked from 'package:base':
strsplit
Loading required package: DECIPHER
Loading required package: RSQLite
Loading required package: parallel
Loading required package: reshape2
Loading required package: phangorn
Loading required package: sangerseqR
Loading required package: gridExtra
Attaching package: 'gridExtra'
The following object is masked from 'package:BiocGenerics':
combine
Loading required package: shiny
Loading required package: shinydashboard
Attaching package: 'shinydashboard'
The following object is masked from 'package:graphics':
box
Loading required package: shinyjs
Attaching package: 'shinyjs'
The following object is masked from 'package:shiny':
runExample
The following object is masked from 'package:RSQLite':
show
The following object is masked from 'package:Biostrings':
show
The following object is masked from 'package:GenomeInfoDb':
show
The following object is masked from 'package:XVector':
show
The following object is masked from 'package:IRanges':
show
The following object is masked from 'package:S4Vectors':
show
The following object is masked from 'package:stats4':
show
The following objects are masked from 'package:methods':
removeClass, show
Loading required package: data.table
Attaching package: 'data.table'
The following objects are masked from 'package:reshape2':
dcast, melt
The following object is masked from 'package:IRanges':
shift
The following objects are masked from 'package:S4Vectors':
first, second
Loading required package: plotly
Loading required package: ggplot2
Attaching package: 'plotly'
The following object is masked from 'package:ggplot2':
last_plot
The following object is masked from 'package:XVector':
slice
The following object is masked from 'package:IRanges':
slice
The following object is masked from 'package:S4Vectors':
rename
The following object is masked from 'package:stats':
filter
The following object is masked from 'package:graphics':
layout
Loading required package: DT
Attaching package: 'DT'
The following objects are masked from 'package:shiny':
dataTableOutput, renderDataTable
Loading required package: zeallot
Loading required package: excelR
Loading required package: shinycssloaders
Loading required package: ggdendro
Loading required package: shinyWidgets
Attaching package: 'shinyWidgets'
The following object is masked from 'package:shinyjs':
alert
Loading required package: openxlsx
Loading required package: tools
Loading required package: rmarkdown
Loading required package: knitr
Loading required package: seqinr
Attaching package: 'seqinr'
The following object is masked from 'package:shiny':
a
The following object is masked from 'package:sangerseqR':
read.abif
The following object is masked from 'package:Biostrings':
translate
The following objects are masked from 'package:ape':
as.alignment, consensus
Loading required package: BiocStyle
Attaching package: 'BiocStyle'
The following objects are masked from 'package:rmarkdown':
html_document, md_document, pdf_document
The following object is masked from 'package:shiny':
markdown
Loading required package: logger
Welcome to sangeranalyseR
>
>
> test_check("sangeranalyseR")
INFO [2022-04-13 02:21:34] #################################################
INFO [2022-04-13 02:21:34] #### Start creating SangerAlignment instance ####
INFO [2022-04-13 02:21:34] #################################################
INFO [2022-04-13 02:21:34] >> You are using Regular Expression Method to group AB1 files!
INFO [2022-04-13 02:21:34] ========================================================
INFO [2022-04-13 02:21:34] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:34] ========================================================
INFO [2022-04-13 02:21:34] >> Contig Name: 'Achl_ACHLO006-09'
SUCCESS [2022-04-13 02:21:35] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:35] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:35] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:35] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:21:36] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:36] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:36] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:36] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:21:36] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.06 secs
SUCCESS [2022-04-13 02:21:37] ==========================================================
SUCCESS [2022-04-13 02:21:37] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:37] ==========================================================
INFO [2022-04-13 02:21:37] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:21:37] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:37] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:38] ========================================================
INFO [2022-04-13 02:21:38] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:38] ========================================================
INFO [2022-04-13 02:21:38] >> Contig Name: 'Achl_ACHLO007-09'
SUCCESS [2022-04-13 02:21:38] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:38] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:38] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:38] >> 'Achl_ACHLO007-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:21:39] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:39] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:39] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:39] >> 'Achl_ACHLO007-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:21:39] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:40] ==========================================================
SUCCESS [2022-04-13 02:21:40] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:40] ==========================================================
INFO [2022-04-13 02:21:40] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:21:40] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:40] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:40] ========================================================
INFO [2022-04-13 02:21:40] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:40] ========================================================
INFO [2022-04-13 02:21:40] >> Contig Name: 'Achl_ACHLO040-09'
SUCCESS [2022-04-13 02:21:41] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:41] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:41] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:41] >> 'Achl_ACHLO040-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:21:41] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:41] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:41] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:41] >> 'Achl_ACHLO040-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:21:41] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:43] ==========================================================
SUCCESS [2022-04-13 02:21:43] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:43] ==========================================================
INFO [2022-04-13 02:21:43] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:21:43] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:43] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:43] ========================================================
INFO [2022-04-13 02:21:43] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:43] ========================================================
INFO [2022-04-13 02:21:43] >> Contig Name: 'Achl_ACHLO041-09'
SUCCESS [2022-04-13 02:21:43] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:43] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:43] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:43] >> 'Achl_ACHLO041-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:21:43] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:43] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:43] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:43] >> 'Achl_ACHLO041-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:21:43] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:45] ==========================================================
SUCCESS [2022-04-13 02:21:45] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:45] ==========================================================
INFO [2022-04-13 02:21:45] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:21:45] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:45] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:45] Aligning consensus reads ...
SUCCESS [2022-04-13 02:21:46] #############################################################
SUCCESS [2022-04-13 02:21:46] ######## 'SangerAlignment' S4 instance is created !! ########
SUCCESS [2022-04-13 02:21:46] #############################################################
INFO [2022-04-13 02:21:46] >> 4 contigs detected from 'regular expression'.
INFO [2022-04-13 02:21:46] >> Contig 'Achl_ACHLO006-09':
INFO [2022-04-13 02:21:46] >> 1 forward reads.
INFO [2022-04-13 02:21:46] >> 1 reverse reads.
INFO [2022-04-13 02:21:46] >> Contig 'Achl_ACHLO007-09':
INFO [2022-04-13 02:21:46] >> 1 forward reads.
INFO [2022-04-13 02:21:46] >> 1 reverse reads.
INFO [2022-04-13 02:21:46] >> Contig 'Achl_ACHLO040-09':
INFO [2022-04-13 02:21:46] >> 1 forward reads.
INFO [2022-04-13 02:21:46] >> 1 reverse reads.
INFO [2022-04-13 02:21:46] >> Contig 'Achl_ACHLO041-09':
INFO [2022-04-13 02:21:46] >> 1 forward reads.
INFO [2022-04-13 02:21:46] >> 1 reverse reads.
INFO [2022-04-13 02:21:46] >> 8 reads created from ABIF file.
INFO [2022-04-13 02:21:46] >> Reads are trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:21:46] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:21:46] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:21:46] #################################################
INFO [2022-04-13 02:21:46] #### Start creating SangerAlignment instance ####
INFO [2022-04-13 02:21:46] #################################################
INFO [2022-04-13 02:21:46] **** You are using Regular Expression Method to group reads in FASTA file (No CSV file)!
INFO [2022-04-13 02:21:46] ========================================================
INFO [2022-04-13 02:21:46] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:46] ========================================================
INFO [2022-04-13 02:21:46] >> Contig Name: 'Achl_ACHLO006-09'
SUCCESS [2022-04-13 02:21:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:46] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:46] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:21:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:46] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:46] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:21:46] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:48] ==========================================================
SUCCESS [2022-04-13 02:21:48] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:48] ==========================================================
INFO [2022-04-13 02:21:48] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:21:48] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:48] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:48] ========================================================
INFO [2022-04-13 02:21:48] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:48] ========================================================
INFO [2022-04-13 02:21:48] >> Contig Name: 'Achl_ACHLO007-09'
SUCCESS [2022-04-13 02:21:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:48] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:48] >> 'Achl_ACHLO007-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:21:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:48] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:48] >> 'Achl_ACHLO007-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:21:48] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:50] ==========================================================
SUCCESS [2022-04-13 02:21:50] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:50] ==========================================================
INFO [2022-04-13 02:21:50] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:21:50] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:50] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:50] ========================================================
INFO [2022-04-13 02:21:50] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:50] ========================================================
INFO [2022-04-13 02:21:50] >> Contig Name: 'Achl_ACHLO040-09'
SUCCESS [2022-04-13 02:21:50] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:50] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:50] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:50] >> 'Achl_ACHLO040-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:21:50] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:50] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:50] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:50] >> 'Achl_ACHLO040-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:21:50] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:52] ==========================================================
SUCCESS [2022-04-13 02:21:52] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:52] ==========================================================
INFO [2022-04-13 02:21:52] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:21:52] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:52] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:52] ========================================================
INFO [2022-04-13 02:21:52] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:52] ========================================================
INFO [2022-04-13 02:21:52] >> Contig Name: 'Achl_ACHLO041-09'
SUCCESS [2022-04-13 02:21:52] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:52] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:52] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:52] >> 'Achl_ACHLO041-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:21:53] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:53] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:53] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:53] >> 'Achl_ACHLO041-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:21:53] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:54] ==========================================================
SUCCESS [2022-04-13 02:21:54] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:54] ==========================================================
INFO [2022-04-13 02:21:54] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:21:54] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:54] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:54] Aligning consensus reads ...
SUCCESS [2022-04-13 02:21:55] #############################################################
SUCCESS [2022-04-13 02:21:55] ######## 'SangerAlignment' S4 instance is created !! ########
SUCCESS [2022-04-13 02:21:55] #############################################################
INFO [2022-04-13 02:21:55] >> 4 contigs detected from 'regular expression'.
INFO [2022-04-13 02:21:55] >> Contig 'Achl_ACHLO006-09':
INFO [2022-04-13 02:21:55] >> 1 forward reads.
INFO [2022-04-13 02:21:55] >> 1 reverse reads.
INFO [2022-04-13 02:21:55] >> Contig 'Achl_ACHLO007-09':
INFO [2022-04-13 02:21:55] >> 1 forward reads.
INFO [2022-04-13 02:21:55] >> 1 reverse reads.
INFO [2022-04-13 02:21:55] >> Contig 'Achl_ACHLO040-09':
INFO [2022-04-13 02:21:55] >> 1 forward reads.
INFO [2022-04-13 02:21:55] >> 1 reverse reads.
INFO [2022-04-13 02:21:55] >> Contig 'Achl_ACHLO041-09':
INFO [2022-04-13 02:21:55] >> 1 forward reads.
INFO [2022-04-13 02:21:55] >> 1 reverse reads.
INFO [2022-04-13 02:21:55] >> 8 reads created from FASTA file.
DEBUG [2022-04-13 02:21:55] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:21:55] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:21:55] ========================================================
INFO [2022-04-13 02:21:55] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:55] ========================================================
INFO [2022-04-13 02:21:55] >> Contig Name: 'Achl_ACHLO006-09'
INFO [2022-04-13 02:21:55] >> You are using Regular Expression Method to group AB1 files!
INFO [2022-04-13 02:21:55] >> Your contig name is Achl_ACHLO006-09
SUCCESS [2022-04-13 02:21:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:56] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:56] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:21:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:56] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:56] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:21:56] >> The number of reads detected: 2
INFO [2022-04-13 02:21:56] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:21:57] ==========================================================
SUCCESS [2022-04-13 02:21:57] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:21:57] ==========================================================
INFO [2022-04-13 02:21:57] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:21:57] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:21:57] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:21:57] >> Trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:21:57] >> For more information, please run 'object'
DEBUG [2022-04-13 02:21:57] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:21:57] ========================================================
INFO [2022-04-13 02:21:57] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:21:57] ========================================================
INFO [2022-04-13 02:21:57] >> Contig Name: 'Achl_RBNII384-13'
INFO [2022-04-13 02:21:57] >> You are using Regular Expression Method to group AB1 files!
INFO [2022-04-13 02:21:57] >> Your contig name is Achl_RBNII384-13
SUCCESS [2022-04-13 02:21:58] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:58] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:58] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:58] >> 'Achl_RBNII384-13_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:21:58] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:58] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:21:58] --------------------------------------------------------
SUCCESS [2022-04-13 02:21:58] >> 'Achl_RBNII384-13_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:21:58] >> The number of reads detected: 2
INFO [2022-04-13 02:21:58] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:00] ==========================================================
SUCCESS [2022-04-13 02:22:00] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:00] ==========================================================
INFO [2022-04-13 02:22:00] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:22:00] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:00] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:00] >> Trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:22:00] >> For more information, please run 'object'
DEBUG [2022-04-13 02:22:00] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:22:00] ========================================================
INFO [2022-04-13 02:22:00] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:00] ========================================================
INFO [2022-04-13 02:22:00] >> Contig Name: 'Achl_ACHLO006-09'
INFO [2022-04-13 02:22:00] >> You are using Regular Expression Method to group reads in FASTA file (No CSV file)!
INFO [2022-04-13 02:22:00] >> Your contig name is Achl_ACHLO006-09
SUCCESS [2022-04-13 02:22:00] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:00] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:00] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:00] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:22:00] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:00] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:00] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:00] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:22:00] >> The number of reads detected: 2
INFO [2022-04-13 02:22:00] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:02] ==========================================================
SUCCESS [2022-04-13 02:22:02] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:02] ==========================================================
INFO [2022-04-13 02:22:02] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:22:02] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:02] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
DEBUG [2022-04-13 02:22:02] >> For more information, please run 'object'
DEBUG [2022-04-13 02:22:02] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:22:02] ========================================================
INFO [2022-04-13 02:22:02] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:02] ========================================================
INFO [2022-04-13 02:22:02] >> Contig Name: 'Achl_RBNII384-13'
INFO [2022-04-13 02:22:02] >> You are using CSV Name Conversion Method to group reads in FASTA file (with Csv file)!
INFO [2022-04-13 02:22:02] >> Your contig name is Achl_RBNII384-13
SUCCESS [2022-04-13 02:22:02] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:02] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:02] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:02] >> 'Achl_RBNII384-13_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:22:02] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:02] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:02] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:02] >> 'Achl_RBNII384-13_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:22:02] >> The number of reads detected: 2
INFO [2022-04-13 02:22:02] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:04] ==========================================================
SUCCESS [2022-04-13 02:22:04] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:04] ==========================================================
INFO [2022-04-13 02:22:04] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:22:04] >> 1 reads assigned to 'forward reads' according to 'csv file'.
INFO [2022-04-13 02:22:04] >> 1 reads assigned to 'reverse reads' according to 'csv file'.
DEBUG [2022-04-13 02:22:04] >> For more information, please run 'object'
DEBUG [2022-04-13 02:22:04] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:22:04] ------------------------------------------------
INFO [2022-04-13 02:22:04] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:22:04] ------------------------------------------------
INFO [2022-04-13 02:22:04] >> Forward Read: Creating abif & sangerseq ...
INFO [2022-04-13 02:22:04] >> Creating Forward Read raw abif ...
INFO [2022-04-13 02:22:04] >> Creating Forward Read raw sangerseq ...
INFO [2022-04-13 02:22:04] * Making basecall !!
INFO [2022-04-13 02:22:04] * Updating slots in 'SangerRead' instance !!
SUCCESS [2022-04-13 02:22:04] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:04] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:04] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:04] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF).
INFO [2022-04-13 02:22:04] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:22:04] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:22:04] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
INFO [2022-04-13 02:22:04] ------------------------------------------------
INFO [2022-04-13 02:22:04] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:22:04] ------------------------------------------------
INFO [2022-04-13 02:22:04] >> Reverse Read: Creating abif & sangerseq ...
INFO [2022-04-13 02:22:04] >> Creating Reverse Read raw abif ...
INFO [2022-04-13 02:22:04] >> Creating Reverse Read raw sangerseq ...
INFO [2022-04-13 02:22:04] * Making basecall !!
INFO [2022-04-13 02:22:04] * Updating slots in 'SangerRead' instance !!
SUCCESS [2022-04-13 02:22:05] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:05] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:05] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:05] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:22:05] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:22:05] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:22:05] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
INFO [2022-04-13 02:22:05] ------------------------------------------------
INFO [2022-04-13 02:22:05] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:22:05] ------------------------------------------------
INFO [2022-04-13 02:22:05] Forward Read: Creating SangerRead from FASTA ...
SUCCESS [2022-04-13 02:22:05] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:05] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:05] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:05] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA).
INFO [2022-04-13 02:22:05] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:22:05] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:22:05] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
INFO [2022-04-13 02:22:05] ------------------------------------------------
INFO [2022-04-13 02:22:05] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:22:05] ------------------------------------------------
INFO [2022-04-13 02:22:05] Reverse Read: Creating SangerRead from FASTA ...
SUCCESS [2022-04-13 02:22:05] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:05] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:05] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:05] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:22:05] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:22:05] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:22:05] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
ERROR [2022-04-13 02:22:05] FILE_NOT_EXIST_ERROR
'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/Allolobophora_chlorotica/ACHLO/TEST_Achl_ACHLO006-09_2_R.ab1' file does not exist.
DEBUG [2022-04-13 02:22:05] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:22:05] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
INFO [2022-04-13 02:22:13] Aligning consensus reads ...
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
INFO [2022-04-13 02:22:22] Aligning consensus reads ...
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
INFO [2022-04-13 02:22:27] * Making basecall !!
INFO [2022-04-13 02:22:27] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:22:27] * Making basecall !!
INFO [2022-04-13 02:22:27] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:22:27] * Making basecall !!
INFO [2022-04-13 02:22:28] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:22:28] * Making basecall !!
INFO [2022-04-13 02:22:28] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:22:28] * Making basecall !!
INFO [2022-04-13 02:22:28] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:22:31] >>> outputDir : C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpE3W0oH
INFO [2022-04-13 02:22:31] Start writing 'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/Allolobophora_chlorotica/ACHLO/Achl_ACHLO006-09_1_F.ab1' to FASTA format ...
INFO [2022-04-13 02:22:31] >> 'C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpE3W0oH/Achl_ACHLO006-09_1_F.fa' is written
INFO [2022-04-13 02:22:31] >>> outputDir : C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpE3W0oH
INFO [2022-04-13 02:22:31] Start writing 'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/fasta/SangerRead/Achl_ACHLO006-09_1_F.fa' to FASTA format ...
INFO [2022-04-13 02:22:31] >> 'C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpE3W0oH/Achl_ACHLO006-09_1_F.fa' is written
INFO [2022-04-13 02:22:31] >>> outputDir : C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpE3W0oH
INFO [2022-04-13 02:22:31] Start writing 'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/fasta/SangerRead/Achl_ACHLO006-09_2_R.fa' to FASTA format ...
INFO [2022-04-13 02:22:31] >> 'C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpE3W0oH/Achl_ACHLO006-09_2_R.fa' is written
INFO [2022-04-13 02:22:31] Aligning consensus reads ...
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.02 secs
INFO [2022-04-13 02:22:33] * Making basecall !!
INFO [2022-04-13 02:22:33] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:22:33] * Making basecall !!
INFO [2022-04-13 02:22:34] * Updating slots in 'SangerRead' instance !!
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 899 ]
>
> proc.time()
user system elapsed
65.50 1.15 66.64
|
sangeranalyseR.Rcheck/tests_x64/testthat.Rout
R version 4.1.3 (2022-03-10) -- "One Push-Up"
Copyright (C) 2022 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(sangeranalyseR)
Loading required package: stringr
Loading required package: ape
Loading required package: Biostrings
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
tapply, union, unique, unsplit, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Attaching package: 'IRanges'
The following object is masked from 'package:grDevices':
windows
Loading required package: XVector
Loading required package: GenomeInfoDb
Attaching package: 'Biostrings'
The following object is masked from 'package:ape':
complement
The following object is masked from 'package:base':
strsplit
Loading required package: DECIPHER
Loading required package: RSQLite
Loading required package: parallel
Loading required package: reshape2
Loading required package: phangorn
Loading required package: sangerseqR
Loading required package: gridExtra
Attaching package: 'gridExtra'
The following object is masked from 'package:BiocGenerics':
combine
Loading required package: shiny
Loading required package: shinydashboard
Attaching package: 'shinydashboard'
The following object is masked from 'package:graphics':
box
Loading required package: shinyjs
Attaching package: 'shinyjs'
The following object is masked from 'package:shiny':
runExample
The following object is masked from 'package:RSQLite':
show
The following object is masked from 'package:Biostrings':
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show
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show
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show
The following object is masked from 'package:S4Vectors':
show
The following object is masked from 'package:stats4':
show
The following objects are masked from 'package:methods':
removeClass, show
Loading required package: data.table
Attaching package: 'data.table'
The following objects are masked from 'package:reshape2':
dcast, melt
The following object is masked from 'package:IRanges':
shift
The following objects are masked from 'package:S4Vectors':
first, second
Loading required package: plotly
Loading required package: ggplot2
Attaching package: 'plotly'
The following object is masked from 'package:ggplot2':
last_plot
The following object is masked from 'package:XVector':
slice
The following object is masked from 'package:IRanges':
slice
The following object is masked from 'package:S4Vectors':
rename
The following object is masked from 'package:stats':
filter
The following object is masked from 'package:graphics':
layout
Loading required package: DT
Attaching package: 'DT'
The following objects are masked from 'package:shiny':
dataTableOutput, renderDataTable
Loading required package: zeallot
Loading required package: excelR
Loading required package: shinycssloaders
Loading required package: ggdendro
Loading required package: shinyWidgets
Attaching package: 'shinyWidgets'
The following object is masked from 'package:shinyjs':
alert
Loading required package: openxlsx
Loading required package: tools
Loading required package: rmarkdown
Loading required package: knitr
Loading required package: seqinr
Attaching package: 'seqinr'
The following object is masked from 'package:shiny':
a
The following object is masked from 'package:sangerseqR':
read.abif
The following object is masked from 'package:Biostrings':
translate
The following objects are masked from 'package:ape':
as.alignment, consensus
Loading required package: BiocStyle
Attaching package: 'BiocStyle'
The following objects are masked from 'package:rmarkdown':
html_document, md_document, pdf_document
The following object is masked from 'package:shiny':
markdown
Loading required package: logger
Welcome to sangeranalyseR
>
>
> test_check("sangeranalyseR")
INFO [2022-04-13 02:22:41] #################################################
INFO [2022-04-13 02:22:41] #### Start creating SangerAlignment instance ####
INFO [2022-04-13 02:22:41] #################################################
INFO [2022-04-13 02:22:41] >> You are using Regular Expression Method to group AB1 files!
INFO [2022-04-13 02:22:41] ========================================================
INFO [2022-04-13 02:22:41] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:41] ========================================================
INFO [2022-04-13 02:22:41] >> Contig Name: 'Achl_ACHLO006-09'
SUCCESS [2022-04-13 02:22:42] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:42] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:42] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:42] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:22:43] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:43] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:43] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:43] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:22:43] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
SUCCESS [2022-04-13 02:22:45] ==========================================================
SUCCESS [2022-04-13 02:22:45] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:45] ==========================================================
INFO [2022-04-13 02:22:45] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:22:45] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:45] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:45] ========================================================
INFO [2022-04-13 02:22:45] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:45] ========================================================
INFO [2022-04-13 02:22:45] >> Contig Name: 'Achl_ACHLO007-09'
SUCCESS [2022-04-13 02:22:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:46] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:46] >> 'Achl_ACHLO007-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:22:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:46] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:46] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:46] >> 'Achl_ACHLO007-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:22:46] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:47] ==========================================================
SUCCESS [2022-04-13 02:22:47] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:47] ==========================================================
INFO [2022-04-13 02:22:47] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:22:47] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:47] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:47] ========================================================
INFO [2022-04-13 02:22:47] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:47] ========================================================
INFO [2022-04-13 02:22:47] >> Contig Name: 'Achl_ACHLO040-09'
SUCCESS [2022-04-13 02:22:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:48] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:48] >> 'Achl_ACHLO040-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:22:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:48] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:48] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:48] >> 'Achl_ACHLO040-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:22:48] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
SUCCESS [2022-04-13 02:22:50] ==========================================================
SUCCESS [2022-04-13 02:22:50] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:50] ==========================================================
INFO [2022-04-13 02:22:50] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:22:50] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:50] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:50] ========================================================
INFO [2022-04-13 02:22:50] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:50] ========================================================
INFO [2022-04-13 02:22:50] >> Contig Name: 'Achl_ACHLO041-09'
SUCCESS [2022-04-13 02:22:50] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:50] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:50] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:50] >> 'Achl_ACHLO041-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:22:51] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:51] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:51] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:51] >> 'Achl_ACHLO041-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:22:51] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:52] ==========================================================
SUCCESS [2022-04-13 02:22:52] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:52] ==========================================================
INFO [2022-04-13 02:22:52] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:22:52] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:52] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:52] Aligning consensus reads ...
SUCCESS [2022-04-13 02:22:53] #############################################################
SUCCESS [2022-04-13 02:22:53] ######## 'SangerAlignment' S4 instance is created !! ########
SUCCESS [2022-04-13 02:22:53] #############################################################
INFO [2022-04-13 02:22:53] >> 4 contigs detected from 'regular expression'.
INFO [2022-04-13 02:22:53] >> Contig 'Achl_ACHLO006-09':
INFO [2022-04-13 02:22:53] >> 1 forward reads.
INFO [2022-04-13 02:22:53] >> 1 reverse reads.
INFO [2022-04-13 02:22:53] >> Contig 'Achl_ACHLO007-09':
INFO [2022-04-13 02:22:53] >> 1 forward reads.
INFO [2022-04-13 02:22:53] >> 1 reverse reads.
INFO [2022-04-13 02:22:53] >> Contig 'Achl_ACHLO040-09':
INFO [2022-04-13 02:22:53] >> 1 forward reads.
INFO [2022-04-13 02:22:53] >> 1 reverse reads.
INFO [2022-04-13 02:22:53] >> Contig 'Achl_ACHLO041-09':
INFO [2022-04-13 02:22:53] >> 1 forward reads.
INFO [2022-04-13 02:22:53] >> 1 reverse reads.
INFO [2022-04-13 02:22:53] >> 8 reads created from ABIF file.
INFO [2022-04-13 02:22:53] >> Reads are trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:22:53] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:22:53] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:22:53] #################################################
INFO [2022-04-13 02:22:53] #### Start creating SangerAlignment instance ####
INFO [2022-04-13 02:22:53] #################################################
INFO [2022-04-13 02:22:53] **** You are using Regular Expression Method to group reads in FASTA file (No CSV file)!
INFO [2022-04-13 02:22:53] ========================================================
INFO [2022-04-13 02:22:53] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:53] ========================================================
INFO [2022-04-13 02:22:53] >> Contig Name: 'Achl_ACHLO006-09'
SUCCESS [2022-04-13 02:22:54] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:54] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:54] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:54] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:22:54] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:54] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:54] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:54] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:22:54] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:55] ==========================================================
SUCCESS [2022-04-13 02:22:55] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:55] ==========================================================
INFO [2022-04-13 02:22:55] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:22:55] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:55] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:55] ========================================================
INFO [2022-04-13 02:22:55] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:55] ========================================================
INFO [2022-04-13 02:22:55] >> Contig Name: 'Achl_ACHLO007-09'
SUCCESS [2022-04-13 02:22:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:56] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:56] >> 'Achl_ACHLO007-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:22:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:56] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:56] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:56] >> 'Achl_ACHLO007-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:22:56] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:22:58] ==========================================================
SUCCESS [2022-04-13 02:22:58] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:22:58] ==========================================================
INFO [2022-04-13 02:22:58] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:22:58] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:22:58] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:22:58] ========================================================
INFO [2022-04-13 02:22:58] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:22:58] ========================================================
INFO [2022-04-13 02:22:58] >> Contig Name: 'Achl_ACHLO040-09'
SUCCESS [2022-04-13 02:22:58] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:58] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:58] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:58] >> 'Achl_ACHLO040-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:22:59] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:59] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:22:59] --------------------------------------------------------
SUCCESS [2022-04-13 02:22:59] >> 'Achl_ACHLO040-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:22:59] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
SUCCESS [2022-04-13 02:23:00] ==========================================================
SUCCESS [2022-04-13 02:23:00] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:23:00] ==========================================================
INFO [2022-04-13 02:23:00] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:23:00] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:23:00] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:23:00] ========================================================
INFO [2022-04-13 02:23:00] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:23:00] ========================================================
INFO [2022-04-13 02:23:00] >> Contig Name: 'Achl_ACHLO041-09'
SUCCESS [2022-04-13 02:23:00] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:00] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:00] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:00] >> 'Achl_ACHLO041-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:23:01] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:01] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:01] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:01] >> 'Achl_ACHLO041-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:23:01] >> The number of reads detected: 2
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:23:02] ==========================================================
SUCCESS [2022-04-13 02:23:02] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:23:02] ==========================================================
INFO [2022-04-13 02:23:02] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:23:02] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:23:02] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:23:02] Aligning consensus reads ...
SUCCESS [2022-04-13 02:23:03] #############################################################
SUCCESS [2022-04-13 02:23:03] ######## 'SangerAlignment' S4 instance is created !! ########
SUCCESS [2022-04-13 02:23:03] #############################################################
INFO [2022-04-13 02:23:03] >> 4 contigs detected from 'regular expression'.
INFO [2022-04-13 02:23:03] >> Contig 'Achl_ACHLO006-09':
INFO [2022-04-13 02:23:03] >> 1 forward reads.
INFO [2022-04-13 02:23:03] >> 1 reverse reads.
INFO [2022-04-13 02:23:03] >> Contig 'Achl_ACHLO007-09':
INFO [2022-04-13 02:23:03] >> 1 forward reads.
INFO [2022-04-13 02:23:03] >> 1 reverse reads.
INFO [2022-04-13 02:23:03] >> Contig 'Achl_ACHLO040-09':
INFO [2022-04-13 02:23:03] >> 1 forward reads.
INFO [2022-04-13 02:23:03] >> 1 reverse reads.
INFO [2022-04-13 02:23:03] >> Contig 'Achl_ACHLO041-09':
INFO [2022-04-13 02:23:03] >> 1 forward reads.
INFO [2022-04-13 02:23:03] >> 1 reverse reads.
INFO [2022-04-13 02:23:03] >> 8 reads created from FASTA file.
DEBUG [2022-04-13 02:23:03] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:23:03] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:23:03] ========================================================
INFO [2022-04-13 02:23:03] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:23:03] ========================================================
INFO [2022-04-13 02:23:03] >> Contig Name: 'Achl_ACHLO006-09'
INFO [2022-04-13 02:23:03] >> You are using Regular Expression Method to group AB1 files!
INFO [2022-04-13 02:23:03] >> Your contig name is Achl_ACHLO006-09
SUCCESS [2022-04-13 02:23:03] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:03] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:03] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:03] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:23:04] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:04] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:04] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:04] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:23:04] >> The number of reads detected: 2
INFO [2022-04-13 02:23:04] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:23:05] ==========================================================
SUCCESS [2022-04-13 02:23:05] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:23:05] ==========================================================
INFO [2022-04-13 02:23:05] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:23:05] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:23:05] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:23:05] >> Trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:23:05] >> For more information, please run 'object'
DEBUG [2022-04-13 02:23:05] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:23:05] ========================================================
INFO [2022-04-13 02:23:05] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:23:05] ========================================================
INFO [2022-04-13 02:23:05] >> Contig Name: 'Achl_RBNII384-13'
INFO [2022-04-13 02:23:05] >> You are using Regular Expression Method to group AB1 files!
INFO [2022-04-13 02:23:05] >> Your contig name is Achl_RBNII384-13
SUCCESS [2022-04-13 02:23:06] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:06] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:06] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:06] >> 'Achl_RBNII384-13_1_F.ab1' is created (Forward Read; ABIF).
SUCCESS [2022-04-13 02:23:06] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:06] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:06] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:06] >> 'Achl_RBNII384-13_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:23:06] >> The number of reads detected: 2
INFO [2022-04-13 02:23:06] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:23:08] ==========================================================
SUCCESS [2022-04-13 02:23:08] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:23:08] ==========================================================
INFO [2022-04-13 02:23:08] >> 2 read(s) created from ABIF file.
INFO [2022-04-13 02:23:08] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:23:08] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
INFO [2022-04-13 02:23:08] >> Trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:23:08] >> For more information, please run 'object'
DEBUG [2022-04-13 02:23:08] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:23:08] ========================================================
INFO [2022-04-13 02:23:08] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:23:08] ========================================================
INFO [2022-04-13 02:23:08] >> Contig Name: 'Achl_ACHLO006-09'
INFO [2022-04-13 02:23:08] >> You are using Regular Expression Method to group reads in FASTA file (No CSV file)!
INFO [2022-04-13 02:23:08] >> Your contig name is Achl_ACHLO006-09
SUCCESS [2022-04-13 02:23:08] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:08] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:08] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:08] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:23:09] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:09] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:09] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:09] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:23:09] >> The number of reads detected: 2
INFO [2022-04-13 02:23:09] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
SUCCESS [2022-04-13 02:23:10] ==========================================================
SUCCESS [2022-04-13 02:23:10] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:23:10] ==========================================================
INFO [2022-04-13 02:23:10] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:23:10] >> 1 reads assigned to 'forward reads' according to 'regular expression'.
INFO [2022-04-13 02:23:10] >> 1 reads assigned to 'reverse reads' according to 'regular expression'.
DEBUG [2022-04-13 02:23:10] >> For more information, please run 'object'
DEBUG [2022-04-13 02:23:10] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:23:10] ========================================================
INFO [2022-04-13 02:23:10] ================ Creating 'SangerContig' ===============
INFO [2022-04-13 02:23:10] ========================================================
INFO [2022-04-13 02:23:10] >> Contig Name: 'Achl_RBNII384-13'
INFO [2022-04-13 02:23:10] >> You are using CSV Name Conversion Method to group reads in FASTA file (with Csv file)!
INFO [2022-04-13 02:23:10] >> Your contig name is Achl_RBNII384-13
SUCCESS [2022-04-13 02:23:10] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:10] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:10] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:10] >> 'Achl_RBNII384-13_1_F' is created (Forward Read; FASTA).
SUCCESS [2022-04-13 02:23:11] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:11] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:11] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:11] >> 'Achl_RBNII384-13_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:23:11] >> The number of reads detected: 2
INFO [2022-04-13 02:23:11] Correcting frameshifts in reads using amino acidreference sequence
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
SUCCESS [2022-04-13 02:23:12] ==========================================================
SUCCESS [2022-04-13 02:23:12] ======== 'SangerContig' S4 instance is created !! ========
SUCCESS [2022-04-13 02:23:12] ==========================================================
INFO [2022-04-13 02:23:12] >> 2 read(s) created from FASTA file.
INFO [2022-04-13 02:23:12] >> 1 reads assigned to 'forward reads' according to 'csv file'.
INFO [2022-04-13 02:23:12] >> 1 reads assigned to 'reverse reads' according to 'csv file'.
DEBUG [2022-04-13 02:23:12] >> For more information, please run 'object'
DEBUG [2022-04-13 02:23:12] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads
INFO [2022-04-13 02:23:12] ------------------------------------------------
INFO [2022-04-13 02:23:12] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:23:12] ------------------------------------------------
INFO [2022-04-13 02:23:12] >> Forward Read: Creating abif & sangerseq ...
INFO [2022-04-13 02:23:12] >> Creating Forward Read raw abif ...
INFO [2022-04-13 02:23:12] >> Creating Forward Read raw sangerseq ...
INFO [2022-04-13 02:23:12] * Making basecall !!
INFO [2022-04-13 02:23:12] * Updating slots in 'SangerRead' instance !!
SUCCESS [2022-04-13 02:23:13] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:13] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:13] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:13] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF).
INFO [2022-04-13 02:23:13] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:23:13] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:23:13] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
INFO [2022-04-13 02:23:13] ------------------------------------------------
INFO [2022-04-13 02:23:13] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:23:13] ------------------------------------------------
INFO [2022-04-13 02:23:13] >> Reverse Read: Creating abif & sangerseq ...
INFO [2022-04-13 02:23:13] >> Creating Reverse Read raw abif ...
INFO [2022-04-13 02:23:13] >> Creating Reverse Read raw sangerseq ...
INFO [2022-04-13 02:23:13] * Making basecall !!
INFO [2022-04-13 02:23:13] * Updating slots in 'SangerRead' instance !!
SUCCESS [2022-04-13 02:23:13] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:13] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:13] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:13] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF).
INFO [2022-04-13 02:23:13] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:23:13] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:23:13] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
INFO [2022-04-13 02:23:13] ------------------------------------------------
INFO [2022-04-13 02:23:13] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:23:13] ------------------------------------------------
INFO [2022-04-13 02:23:13] Forward Read: Creating SangerRead from FASTA ...
SUCCESS [2022-04-13 02:23:13] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:13] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:13] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:13] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA).
INFO [2022-04-13 02:23:13] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:23:13] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:23:13] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
INFO [2022-04-13 02:23:13] ------------------------------------------------
INFO [2022-04-13 02:23:13] -------- Creating 'SangerRead' instance --------
INFO [2022-04-13 02:23:13] ------------------------------------------------
INFO [2022-04-13 02:23:14] Reverse Read: Creating SangerRead from FASTA ...
SUCCESS [2022-04-13 02:23:14] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:14] -------- 'SangerRead' S4 instance is created !! --------
SUCCESS [2022-04-13 02:23:14] --------------------------------------------------------
SUCCESS [2022-04-13 02:23:14] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA).
INFO [2022-04-13 02:23:14] >> Read is trimmed by 'M1 - Motts trimming algorithm'.
DEBUG [2022-04-13 02:23:14] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:23:14] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
ERROR [2022-04-13 02:23:14] FILE_NOT_EXIST_ERROR
'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/Allolobophora_chlorotica/ACHLO/TEST_Achl_ACHLO006-09_2_R.ab1' file does not exist.
DEBUG [2022-04-13 02:23:14] >> For more information, please run 'object'.
DEBUG [2022-04-13 02:23:14] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
INFO [2022-04-13 02:23:22] Aligning consensus reads ...
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
INFO [2022-04-13 02:23:30] Aligning consensus reads ...
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.05 secs
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
INFO [2022-04-13 02:23:35] * Making basecall !!
INFO [2022-04-13 02:23:35] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:23:36] * Making basecall !!
INFO [2022-04-13 02:23:36] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:23:36] * Making basecall !!
INFO [2022-04-13 02:23:36] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:23:37] * Making basecall !!
INFO [2022-04-13 02:23:37] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:23:37] * Making basecall !!
INFO [2022-04-13 02:23:37] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:23:40] >>> outputDir : C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpaWE2AE
INFO [2022-04-13 02:23:40] Start writing 'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/Allolobophora_chlorotica/ACHLO/Achl_ACHLO006-09_1_F.ab1' to FASTA format ...
INFO [2022-04-13 02:23:40] >> 'C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpaWE2AE/Achl_ACHLO006-09_1_F.fa' is written
INFO [2022-04-13 02:23:40] >>> outputDir : C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpaWE2AE
INFO [2022-04-13 02:23:40] Start writing 'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/fasta/SangerRead/Achl_ACHLO006-09_1_F.fa' to FASTA format ...
INFO [2022-04-13 02:23:40] >> 'C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpaWE2AE/Achl_ACHLO006-09_1_F.fa' is written
INFO [2022-04-13 02:23:40] >>> outputDir : C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpaWE2AE
INFO [2022-04-13 02:23:40] Start writing 'C:/Users/biocbuild/bbs-3.14-bioc/R/library/sangeranalyseR/extdata/fasta/SangerRead/Achl_ACHLO006-09_2_R.fa' to FASTA format ...
INFO [2022-04-13 02:23:40] >> 'C:\Users\biocbuild\bbs-3.14-bioc\tmpdir\RtmpaWE2AE/Achl_ACHLO006-09_2_R.fa' is written
INFO [2022-04-13 02:23:40] Aligning consensus reads ...
Assessing frameshifts in nucleotide sequences:
================================================================================
Time difference of 0.03 secs
INFO [2022-04-13 02:23:42] * Making basecall !!
INFO [2022-04-13 02:23:43] * Updating slots in 'SangerRead' instance !!
INFO [2022-04-13 02:23:43] * Making basecall !!
INFO [2022-04-13 02:23:43] * Updating slots in 'SangerRead' instance !!
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 899 ]
>
> proc.time()
user system elapsed
68.35 0.79 69.20
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sangeranalyseR.Rcheck/examples_i386/sangeranalyseR-Ex.timings
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sangeranalyseR.Rcheck/examples_x64/sangeranalyseR-Ex.timings
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