Markus Riester
| Snapshot Date: 2021-10-14 04:50:12 -0400 (Thu, 14 Oct 2021) |
| git_url: https://git.bioconductor.org/packages/PureCN |
| git_branch: RELEASE_3_13 |
| git_last_commit: 1d595f4 |
| git_last_commit_date: 2021-07-01 13:33:13 -0400 (Thu, 01 Jul 2021) |
| Back to Multiple platform build/check report for BioC 3.13 |
|
This page was generated on 2021-10-15 15:06:17 -0400 (Fri, 15 Oct 2021).
|
To the developers/maintainers of the PureCN package: - Please allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/PureCN.git to reflect on this report. See How and When does the builder pull? When will my changes propagate? here for more information. - Make sure to use the following settings in order to reproduce any error or warning you see on this page. |
| Package 1461/2041 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | ||||||||
| PureCN 1.22.2 (landing page) Markus Riester
| nebbiolo1 | Linux (Ubuntu 20.04.2 LTS) / x86_64 | OK | OK | OK | | ||||||||
| tokay2 | Windows Server 2012 R2 Standard / x64 | OK | OK | OK | OK | | ||||||||
| machv2 | macOS 10.14.6 Mojave / x86_64 | OK | OK | OK | OK | | ||||||||
| Package: PureCN |
| Version: 1.22.2 |
| Command: C:\Users\biocbuild\bbs-3.13-bioc\R\bin\R.exe CMD check --force-multiarch --install=check:PureCN.install-out.txt --library=C:\Users\biocbuild\bbs-3.13-bioc\R\library --no-vignettes --timings PureCN_1.22.2.tar.gz |
| StartedAt: 2021-10-15 03:52:35 -0400 (Fri, 15 Oct 2021) |
| EndedAt: 2021-10-15 04:26:43 -0400 (Fri, 15 Oct 2021) |
| EllapsedTime: 2047.9 seconds |
| RetCode: 0 |
| Status: OK |
| CheckDir: PureCN.Rcheck |
| Warnings: 0 |
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###
### Running command:
###
### C:\Users\biocbuild\bbs-3.13-bioc\R\bin\R.exe CMD check --force-multiarch --install=check:PureCN.install-out.txt --library=C:\Users\biocbuild\bbs-3.13-bioc\R\library --no-vignettes --timings PureCN_1.22.2.tar.gz
###
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* using log directory 'C:/Users/biocbuild/bbs-3.13-bioc/meat/PureCN.Rcheck'
* using R version 4.1.1 (2021-08-10)
* using platform: x86_64-w64-mingw32 (64-bit)
* using session charset: ISO8859-1
* using option '--no-vignettes'
* checking for file 'PureCN/DESCRIPTION' ... OK
* checking extension type ... Package
* this is package 'PureCN' version '1.22.2'
* checking package namespace information ... OK
* checking package dependencies ... NOTE
Package which this enhances but not available for checking: 'genomicsdb'
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking whether package 'PureCN' can be installed ... OK
* checking installed package size ... NOTE
installed size is 5.9Mb
sub-directories of 1Mb or more:
extdata 4.0Mb
* checking package directory ... OK
* checking 'build' directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* loading checks for arch 'i386'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* loading checks for arch 'x64'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of 'data' directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking files in 'vignettes' ... OK
* checking examples ...
** running examples for arch 'i386' ... OK
Examples with CPU (user + system) or elapsed time > 5s
user system elapsed
callAmplificationsInLowPurity 51.27 0.05 51.32
segmentationPSCBS 27.70 0.13 27.90
filterIntervals 25.45 0.33 25.82
runAbsoluteCN 22.97 0.04 23.02
segmentationHclust 18.89 0.03 18.93
findFocal 14.64 0.02 14.82
segmentationCBS 11.27 0.00 11.26
processMultipleSamples 6.18 0.03 6.33
annotateTargets 5.46 0.53 6.32
** running examples for arch 'x64' ... OK
Examples with CPU (user + system) or elapsed time > 5s
user system elapsed
callAmplificationsInLowPurity 53.87 0.02 53.89
segmentationPSCBS 28.35 0.08 28.44
filterIntervals 24.80 0.00 24.80
segmentationHclust 20.57 0.06 20.63
runAbsoluteCN 19.89 0.04 19.94
findFocal 13.14 0.03 13.17
segmentationCBS 10.08 0.00 10.08
annotateTargets 6.44 0.16 6.59
processMultipleSamples 6.12 0.00 6.13
calculateTangentNormal 5.45 0.00 5.45
* checking for unstated dependencies in 'tests' ... OK
* checking tests ...
** running tests for arch 'i386' ...
Running 'testthat.R'
OK
** running tests for arch 'x64' ...
Running 'testthat.R'
OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in 'inst/doc' ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE
Status: 2 NOTEs
See
'C:/Users/biocbuild/bbs-3.13-bioc/meat/PureCN.Rcheck/00check.log'
for details.
PureCN.Rcheck/00install.out
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###
### Running command:
###
### C:\cygwin\bin\curl.exe -O http://155.52.207.165/BBS/3.13/bioc/src/contrib/PureCN_1.22.2.tar.gz && rm -rf PureCN.buildbin-libdir && mkdir PureCN.buildbin-libdir && C:\Users\biocbuild\bbs-3.13-bioc\R\bin\R.exe CMD INSTALL --merge-multiarch --build --library=PureCN.buildbin-libdir PureCN_1.22.2.tar.gz && C:\Users\biocbuild\bbs-3.13-bioc\R\bin\R.exe CMD INSTALL PureCN_1.22.2.zip && rm PureCN_1.22.2.tar.gz PureCN_1.22.2.zip
###
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% Total % Received % Xferd Average Speed Time Time Time Current
Dload Upload Total Spent Left Speed
0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0
43 2906k 43 1256k 0 0 1394k 0 0:00:02 --:--:-- 0:00:02 1393k
100 2906k 100 2906k 0 0 1861k 0 0:00:01 0:00:01 --:--:-- 1863k
install for i386
* installing *source* package 'PureCN' ...
** using staged installation
** R
** data
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
converting help for package 'PureCN'
finding HTML links ... done
PureCN-defunct html
PureCN-deprecated html
annotateTargets html
bootstrapResults html
calculateBamCoverageByInterval html
calculateLogRatio html
calculateMappingBiasGatk4 html
calculateMappingBiasVcf html
calculatePowerDetectSomatic html
calculateTangentNormal html
callAlterations html
callAlterationsFromSegmentation html
callAmplificationsInLowPurity html
callCIN html
callLOH html
callMutationBurden html
centromeres html
correctCoverageBias html
createCurationFile html
createNormalDatabase html
filterIntervals html
filterVcfBasic html
filterVcfMuTect html
filterVcfMuTect2 html
findFocal html
getSexFromCoverage html
getSexFromVcf html
plotAbs html
poolCoverage html
predictSomatic html
preprocessIntervals html
processMultipleSamples html
purecn.DNAcopy.bdry html
purecn.example.output html
readAllelicCountsFile html
readCoverageFile html
readCurationFile html
readLogRatioFile html
readSegmentationFile html
runAbsoluteCN html
segmentationCBS html
segmentationGATK4 html
segmentationHclust html
segmentationPSCBS html
setMappingBiasVcf html
setPriorVcf html
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
install for x64
* installing *source* package 'PureCN' ...
** testing if installed package can be loaded
* MD5 sums
packaged installation of 'PureCN' as PureCN_1.22.2.zip
* DONE (PureCN)
* installing to library 'C:/Users/biocbuild/bbs-3.13-bioc/R/library'
package 'PureCN' successfully unpacked and MD5 sums checked
|
PureCN.Rcheck/tests_i386/testthat.Rout
R version 4.1.1 (2021-08-10) -- "Kick Things"
Copyright (C) 2021 The R Foundation for Statistical Computing
Platform: i386-w64-mingw32/i386 (32-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(PureCN)
Loading required package: DNAcopy
Loading required package: VariantAnnotation
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
tapply, union, unique, unsplit, which.max, which.min
Loading required package: MatrixGenerics
Loading required package: matrixStats
Attaching package: 'MatrixGenerics'
The following objects are masked from 'package:matrixStats':
colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
colWeightedMeans, colWeightedMedians, colWeightedSds,
colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
rowWeightedSds, rowWeightedVars
Loading required package: GenomeInfoDb
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Attaching package: 'IRanges'
The following object is masked from 'package:grDevices':
windows
Loading required package: GenomicRanges
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Attaching package: 'Biobase'
The following object is masked from 'package:MatrixGenerics':
rowMedians
The following objects are masked from 'package:matrixStats':
anyMissing, rowMedians
Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
Attaching package: 'VariantAnnotation'
The following object is masked from 'package:base':
tabulate
>
> test_check("PureCN")
WARN [2021-10-15 04:06:12] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
WARN [2021-10-15 04:06:12] Chromosome naming style of txdb file (UCSC) was different from interval file (Ensembl).
WARN [2021-10-15 04:06:16] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
FATAL [2021-10-15 04:06:19] tumor.coverage.file and interval.file do not align.
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:06:19] Need either f or purity and ploidy.
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:06:19] f not in expected range.
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:06:19] coverage not in expected range (>=2)
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:06:19] purity not in expected range.
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:06:19] ploidy not in expected range.
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:06:19] cell.fraction not in expected range.
FATAL [2021-10-15 04:06:19]
FATAL [2021-10-15 04:06:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:06:19] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:06:22] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:06:22] You are likely not using the correct baits file!
WARN [2021-10-15 04:06:22] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:06:22] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:06:22] Processing on-target regions...
INFO [2021-10-15 04:06:23] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:06:23] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:06:26] Tumor/normal noise ratio: 19.041
WARN [2021-10-15 04:06:26] Extensive noise in tumor compared to normals.
INFO [2021-10-15 04:07:25] Tumor/normal noise ratio: 19.041
WARN [2021-10-15 04:07:25] Extensive noise in tumor compared to normals.
INFO [2021-10-15 04:07:26] Using BiocParallel for parallel optimization.
FATAL [2021-10-15 04:08:43] pvalue.cutoff not within expected range or format.
FATAL [2021-10-15 04:08:43]
FATAL [2021-10-15 04:08:43] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:08:43] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:08:43] pvalue.cutoff not within expected range or format.
FATAL [2021-10-15 04:08:43]
FATAL [2021-10-15 04:08:43] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:08:43] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:08:43] percentile.cutoff not in expected range (0 to 100).
FATAL [2021-10-15 04:08:43]
FATAL [2021-10-15 04:08:43] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:08:43] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:08:43] percentile.cutoff not in expected range (0 to 100).
FATAL [2021-10-15 04:08:43]
FATAL [2021-10-15 04:08:43] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:08:43] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:08:43] purity not within expected range or format.
FATAL [2021-10-15 04:08:43]
FATAL [2021-10-15 04:08:43] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:08:43] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:08:43] purity not within expected range or format.
FATAL [2021-10-15 04:08:43]
FATAL [2021-10-15 04:08:43] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:08:43] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:08:45] ------------------------------------------------------------
INFO [2021-10-15 04:08:45] PureCN 1.22.2
INFO [2021-10-15 04:08:45] ------------------------------------------------------------
INFO [2021-10-15 04:08:45] Loading coverage files...
INFO [2021-10-15 04:08:45] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:08:46] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:08:46] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:08:46] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:08:46] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:08:46] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:08:46] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:08:46] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:08:46] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:08:46] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:08:46] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:08:46] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:08:46] Loading VCF...
INFO [2021-10-15 04:08:46] Found 127 variants in VCF file.
INFO [2021-10-15 04:08:46] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:08:46] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:08:46] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:08:46] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:08:47] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:08:47] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:08:47] Total size of targeted genomic region: 1.53Mb (2.33Mb with 50bp padding).
INFO [2021-10-15 04:08:47] 1.2% of targets contain variants.
INFO [2021-10-15 04:08:47] Removing 4 variants outside intervals.
INFO [2021-10-15 04:08:47] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:08:47] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:08:48] Found SOMATIC annotation in VCF. Setting mapping bias to 0.972.
INFO [2021-10-15 04:08:48] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:08:48] Sample sex: ?
INFO [2021-10-15 04:08:48] Segmenting data...
INFO [2021-10-15 04:08:48] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:08:48] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:08:49] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:08:49] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:08:50] Using 121 variants.
INFO [2021-10-15 04:08:50] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:08:50] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:08:52] Local optima: 0.63/1.9, 0.5/2
INFO [2021-10-15 04:08:52] Testing local optimum 1/2 at purity 0.63 and total ploidy 1.90...
INFO [2021-10-15 04:08:54] Testing local optimum 2/2 at purity 0.50 and total ploidy 2.00...
INFO [2021-10-15 04:08:55] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:08:55] Fitting variants with beta model for local optimum 1/2...
INFO [2021-10-15 04:08:55] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:08:58] Optimized purity: 0.65
INFO [2021-10-15 04:08:58] Done.
INFO [2021-10-15 04:08:58] ------------------------------------------------------------
INFO [2021-10-15 04:08:59] Estimating callable regions.
FATAL [2021-10-15 04:09:03] exclude not a GRanges object.
FATAL [2021-10-15 04:09:03]
FATAL [2021-10-15 04:09:03] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:03] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:09:03] callable not a GRanges object.
FATAL [2021-10-15 04:09:03]
FATAL [2021-10-15 04:09:03] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:03] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:09:17] tumor.coverage.file and interval.file do not align.
FATAL [2021-10-15 04:09:20] No gc_bias column in interval.file.
FATAL [2021-10-15 04:09:20]
FATAL [2021-10-15 04:09:20] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:20] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:09:20] Provided coverage is zero, most likely due to a corrupt BAM file.
FATAL [2021-10-15 04:09:20]
FATAL [2021-10-15 04:09:20] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:20] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:09:29] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
INFO [2021-10-15 04:09:29] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
INFO [2021-10-15 04:09:29] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
INFO [2021-10-15 04:09:29] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
INFO [2021-10-15 04:09:30] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
INFO [2021-10-15 04:09:30] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
FATAL [2021-10-15 04:09:30] Purity or Ploidy not numeric or in expected range.
FATAL [2021-10-15 04:09:30]
FATAL [2021-10-15 04:09:30] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:30] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:09:30] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
INFO [2021-10-15 04:09:30] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.rds...
FATAL [2021-10-15 04:09:30] 'Failed' column in
FATAL [2021-10-15 04:09:30] C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec57363890.csv
FATAL [2021-10-15 04:09:30] not logical(1).
FATAL [2021-10-15 04:09:30]
FATAL [2021-10-15 04:09:30] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:30] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:09:30] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:09:30] You are likely not using the correct baits file!
WARN [2021-10-15 04:09:31] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:09:31] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:09:31] Processing on-target regions...
INFO [2021-10-15 04:09:31] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:09:31] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:09:36] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:09:36] You are likely not using the correct baits file!
WARN [2021-10-15 04:09:36] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:09:36] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:09:36] Processing on-target regions...
INFO [2021-10-15 04:09:37] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:09:37] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:09:40] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:09:40] You are likely not using the correct baits file!
WARN [2021-10-15 04:09:40] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:09:40] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:09:40] Processing on-target regions...
INFO [2021-10-15 04:09:40] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:09:40] Removing 1 intervals with zero coverage in more than 3% of normalDB.
WARN [2021-10-15 04:09:42] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:09:42] Sample sex: NA
WARN [2021-10-15 04:09:42] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:09:42] Sample sex: NA
INFO [2021-10-15 04:09:44] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:09:44] You are likely not using the correct baits file!
WARN [2021-10-15 04:09:44] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:09:44] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:09:44] Length of normal.coverage.files and sex different
FATAL [2021-10-15 04:09:44]
FATAL [2021-10-15 04:09:44] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:44] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:09:51] Target intervals were not sorted.
INFO [2021-10-15 04:09:52] 560 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:09:52] You are likely not using the correct baits file!
WARN [2021-10-15 04:09:52] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:09:52] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:09:52] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:09:52] Processing on-target regions...
INFO [2021-10-15 04:09:53] Removing 978 intervals with low coverage in normalDB.
INFO [2021-10-15 04:09:53] Removing 11 intervals with zero coverage in more than 3% of normalDB.
FATAL [2021-10-15 04:09:59] tumor.coverage.file and normalDB do not align.
FATAL [2021-10-15 04:09:59]
FATAL [2021-10-15 04:09:59] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:59] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:09:59] At least 2 normal.coverage.files required.
FATAL [2021-10-15 04:09:59]
FATAL [2021-10-15 04:09:59] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:09:59] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:10:04] ------------------------------------------------------------
INFO [2021-10-15 04:10:04] PureCN 1.22.2
INFO [2021-10-15 04:10:04] ------------------------------------------------------------
INFO [2021-10-15 04:10:04] Loading coverage files...
INFO [2021-10-15 04:10:05] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:10:05] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:10:05] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:10:05] Removing 228 intervals with missing log.ratio.
FATAL [2021-10-15 04:10:05] normalDB incompatible with this PureCN version. Please re-run
FATAL [2021-10-15 04:10:05] NormalDB.R.
FATAL [2021-10-15 04:10:05]
FATAL [2021-10-15 04:10:05] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:05] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:10:05] normalDB incompatible with this PureCN version. Please re-run
FATAL [2021-10-15 04:10:05] NormalDB.R.
FATAL [2021-10-15 04:10:05]
FATAL [2021-10-15 04:10:05] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:05] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:10:05] normal.coverage.files with _coverage.txt and _loess.txt suffix
FATAL [2021-10-15 04:10:05] provided. Provide either only GC-normalized or raw coverage files!
FATAL [2021-10-15 04:10:05]
FATAL [2021-10-15 04:10:05] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:05] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:10:06] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:07] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:07] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:07] Removing 16 low quality variants with BQ < 25.
INFO [2021-10-15 04:10:07] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:08] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:08] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:08] Removing 6 blacklisted variants.
INFO [2021-10-15 04:10:08] Removing 16 low quality variants with BQ < 25.
INFO [2021-10-15 04:10:09] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:09] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:09] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:09] Removing 16 low quality variants with BQ < 25.
WARN [2021-10-15 04:10:09] MuTect stats file lacks contig and position columns.
INFO [2021-10-15 04:10:10] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:10] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:10] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:10] Removing 16 low quality variants with BQ < 25.
WARN [2021-10-15 04:10:10] MuTect stats file and VCF file do not align perfectly. Will remove 2325 unmatched variants.
WARN [2021-10-15 04:10:10] MuTect stats file lacks failure_reasons column. Keeping all variants listed in stats file.
INFO [2021-10-15 04:10:11] Removing 0 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:11] Removing 0 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:11] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:10:11] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:12] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:12] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:12] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:13] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:13] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:10:13] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:10:13] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:10:14] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
FATAL [2021-10-15 04:10:14] No variants passed filter BQ.
FATAL [2021-10-15 04:10:14]
FATAL [2021-10-15 04:10:14] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:14] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:10:14] Found 11 variants in VCF file.
WARN [2021-10-15 04:10:15] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2021-10-15 04:10:15] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:10:18] Found 11 variants in VCF file.
WARN [2021-10-15 04:10:18] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2021-10-15 04:10:18] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:10:20] Found 11 variants in VCF file.
WARN [2021-10-15 04:10:20] vcf.file has no DB or POP_AF info field for membership in germline databases. Guessing it based on available dbSNP ID.
INFO [2021-10-15 04:10:21] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:10:22] Found 1000 variants in VCF file.
INFO [2021-10-15 04:10:22] Removing 2 triallelic sites.
WARN [2021-10-15 04:10:22] Having trouble guessing SOMATIC status...
WARN [2021-10-15 04:10:22] DP FORMAT field contains NAs. Removing 44 variants.
INFO [2021-10-15 04:10:22] 954 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:10:26] Found 12 variants in VCF file.
INFO [2021-10-15 04:10:26] Removing 1 triallelic sites.
WARN [2021-10-15 04:10:26] DP FORMAT field contains NAs. Removing 1 variants.
WARN [2021-10-15 04:10:27] vcf.file has no DB info field for membership in germline databases. Found and used somatic status instead.
INFO [2021-10-15 04:10:27] 8 (80.0%) variants annotated as likely germline (DB INFO flag).
WARN [2021-10-15 04:10:28] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:10:29] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:10:29] Mean coverages: chrX: 108.58, chrY: 108.58, chr1-22: 104.87.
INFO [2021-10-15 04:10:29] Mean coverages: chrX: 108.58, chrY: 2.17, chr1-22: 104.87.
INFO [2021-10-15 04:10:29] Mean coverages: chrX: 108.58, chrY: 5.17, chr1-22: 104.87.
INFO [2021-10-15 04:10:29] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:10:30] No germline variants in VCF.
FATAL [2021-10-15 04:10:30] No solution with id hello
FATAL [2021-10-15 04:10:30]
FATAL [2021-10-15 04:10:30] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:30] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:10:30] No solution with id 100
FATAL [2021-10-15 04:10:30]
FATAL [2021-10-15 04:10:30] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:30] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:10:32] all.data and w have different lengths.
FATAL [2021-10-15 04:10:32]
FATAL [2021-10-15 04:10:32] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:32] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:10:33] No mappability scores provided.
WARN [2021-10-15 04:10:33] No reptiming scores provided.
INFO [2021-10-15 04:10:33] Calculating GC-content...
WARN [2021-10-15 04:10:33] No mappability scores provided.
WARN [2021-10-15 04:10:34] No reptiming scores provided.
INFO [2021-10-15 04:10:34] Calculating GC-content...
INFO [2021-10-15 04:10:34] Removing 1 targets overlapping with exclude.
WARN [2021-10-15 04:10:34] No mappability scores provided.
WARN [2021-10-15 04:10:34] No reptiming scores provided.
INFO [2021-10-15 04:10:34] Calculating GC-content...
WARN [2021-10-15 04:10:34] Found small target regions (< 100bp). Will resize them.
FATAL [2021-10-15 04:10:34] off.target.padding must be negative.
FATAL [2021-10-15 04:10:34]
FATAL [2021-10-15 04:10:34] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:34] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:10:35] Interval coordinates should start at 1, not at 0
FATAL [2021-10-15 04:10:35]
FATAL [2021-10-15 04:10:35] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:35] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:10:35] Found small target regions (< 100bp). Will resize them.
FATAL [2021-10-15 04:10:35] No off-target regions after filtering for mappability and
FATAL [2021-10-15 04:10:35] off.target.padding
FATAL [2021-10-15 04:10:35]
FATAL [2021-10-15 04:10:35] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:35] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:10:36] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:10:36] No mappability scores provided.
INFO [2021-10-15 04:10:36] Calculating GC-content...
WARN [2021-10-15 04:10:36] Found small target regions (< 100bp). Will resize them.
INFO [2021-10-15 04:10:37] Averaging reptiming into bins of size 200...
WARN [2021-10-15 04:10:37] No mappability scores provided.
INFO [2021-10-15 04:10:37] Calculating GC-content...
WARN [2021-10-15 04:10:37] Found small target regions (< 100bp). Will resize them.
INFO [2021-10-15 04:10:38] Splitting 1 large targets to an average width of 200.
WARN [2021-10-15 04:10:38] No mappability scores provided.
WARN [2021-10-15 04:10:38] No reptiming scores provided.
INFO [2021-10-15 04:10:38] Calculating GC-content...
WARN [2021-10-15 04:10:38] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:10:38] No mappability scores provided.
WARN [2021-10-15 04:10:38] No reptiming scores provided.
INFO [2021-10-15 04:10:38] Calculating GC-content...
INFO [2021-10-15 04:10:39] Tiling off-target regions to an average width of 200000.
WARN [2021-10-15 04:10:39] No mappability scores provided.
WARN [2021-10-15 04:10:39] No reptiming scores provided.
INFO [2021-10-15 04:10:39] Calculating GC-content...
WARN [2021-10-15 04:10:39] Intervals contain off-target regions. Will not change intervals.
WARN [2021-10-15 04:10:39] No mappability scores provided.
WARN [2021-10-15 04:10:39] No reptiming scores provided.
INFO [2021-10-15 04:10:40] Calculating GC-content...
WARN [2021-10-15 04:10:40] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:10:40] No reptiming scores provided.
INFO [2021-10-15 04:10:40] Calculating GC-content...
WARN [2021-10-15 04:10:41] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:10:42] 1 intervals without mappability score (1 on-target).
INFO [2021-10-15 04:10:42] Removing 1 intervals with low mappability score (<0.60).
WARN [2021-10-15 04:10:42] No reptiming scores provided.
INFO [2021-10-15 04:10:42] Calculating GC-content...
WARN [2021-10-15 04:10:42] Found small target regions (< 100bp). Will resize them.
INFO [2021-10-15 04:10:43] Tiling off-target regions to an average width of 200000.
WARN [2021-10-15 04:10:43] No reptiming scores provided.
INFO [2021-10-15 04:10:43] Calculating GC-content...
WARN [2021-10-15 04:10:44] No mappability scores provided.
WARN [2021-10-15 04:10:44] No reptiming scores provided.
INFO [2021-10-15 04:10:44] Calculating GC-content...
WARN [2021-10-15 04:10:45] Chromosome naming style of interval file (Ensembl) was different from reference (UCSC).
WARN [2021-10-15 04:10:45] No mappability scores provided.
WARN [2021-10-15 04:10:45] No reptiming scores provided.
INFO [2021-10-15 04:10:45] Calculating GC-content...
FATAL [2021-10-15 04:10:45] Chromosome naming style of interval file unknown, should be UCSC.
FATAL [2021-10-15 04:10:45]
FATAL [2021-10-15 04:10:45] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:10:45] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:10:45] Chromosome naming style of interval file (Ensembl) was different from reference (UCSC).
WARN [2021-10-15 04:10:46] Chromosome naming style of mappability file (Ensembl) was different from reference (UCSC).
WARN [2021-10-15 04:10:46] No reptiming scores provided.
INFO [2021-10-15 04:10:46] Calculating GC-content...
WARN [2021-10-15 04:10:46] Found small target regions (< 60bp). Will resize them.
WARN [2021-10-15 04:10:47] No mappability scores provided.
WARN [2021-10-15 04:10:47] No reptiming scores provided.
INFO [2021-10-15 04:10:47] Calculating GC-content...
WARN [2021-10-15 04:10:47] Found small target regions (< 60bp). Will drop them.
WARN [2021-10-15 04:10:47] No mappability scores provided.
WARN [2021-10-15 04:10:47] No reptiming scores provided.
INFO [2021-10-15 04:10:47] Calculating GC-content...
WARN [2021-10-15 04:10:47] Found small target regions (< 200bp). Will resize them.
WARN [2021-10-15 04:10:48] No mappability scores provided.
WARN [2021-10-15 04:10:48] No reptiming scores provided.
INFO [2021-10-15 04:10:48] Calculating GC-content...
INFO [2021-10-15 04:10:49] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:10:49] You are likely not using the correct baits file!
WARN [2021-10-15 04:10:49] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:10:49] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:10:49] Processing on-target regions...
INFO [2021-10-15 04:10:50] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:10:50] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:10:55] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
multipcf finished for chromosome arm 1p
multipcf finished for chromosome arm 1q
multipcf finished for chromosome arm 2p
multipcf finished for chromosome arm 2q
multipcf finished for chromosome arm 3p
multipcf finished for chromosome arm 3q
multipcf finished for chromosome arm 4p
multipcf finished for chromosome arm 4q
multipcf finished for chromosome arm 5p
multipcf finished for chromosome arm 5q
multipcf finished for chromosome arm 6p
multipcf finished for chromosome arm 6q
multipcf finished for chromosome arm 7p
multipcf finished for chromosome arm 7q
multipcf finished for chromosome arm 8p
multipcf finished for chromosome arm 8q
multipcf finished for chromosome arm 9p
multipcf finished for chromosome arm 9q
multipcf finished for chromosome arm 10p
multipcf finished for chromosome arm 10q
multipcf finished for chromosome arm 11p
multipcf finished for chromosome arm 11q
multipcf finished for chromosome arm 12p
multipcf finished for chromosome arm 12q
multipcf finished for chromosome arm 13q
multipcf finished for chromosome arm 14q
multipcf finished for chromosome arm 15q
multipcf finished for chromosome arm 16p
multipcf finished for chromosome arm 16q
multipcf finished for chromosome arm 17p
multipcf finished for chromosome arm 17q
multipcf finished for chromosome arm 18p
multipcf finished for chromosome arm 18q
multipcf finished for chromosome arm 19p
multipcf finished for chromosome arm 19q
multipcf finished for chromosome arm 20p
multipcf finished for chromosome arm 20q
multipcf finished for chromosome arm 21q
multipcf finished for chromosome arm 22q
INFO [2021-10-15 04:11:01] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
multipcf finished for chromosome arm 1p
multipcf finished for chromosome arm 1q
multipcf finished for chromosome arm 2p
multipcf finished for chromosome arm 2q
multipcf finished for chromosome arm 3p
multipcf finished for chromosome arm 3q
multipcf finished for chromosome arm 4p
multipcf finished for chromosome arm 4q
multipcf finished for chromosome arm 5p
multipcf finished for chromosome arm 5q
multipcf finished for chromosome arm 6p
multipcf finished for chromosome arm 6q
multipcf finished for chromosome arm 7p
multipcf finished for chromosome arm 7q
multipcf finished for chromosome arm 8p
multipcf finished for chromosome arm 8q
multipcf finished for chromosome arm 9p
multipcf finished for chromosome arm 9q
multipcf finished for chromosome arm 10p
multipcf finished for chromosome arm 10q
multipcf finished for chromosome arm 11p
multipcf finished for chromosome arm 11q
multipcf finished for chromosome arm 12p
multipcf finished for chromosome arm 12q
multipcf finished for chromosome arm 13q
multipcf finished for chromosome arm 14q
multipcf finished for chromosome arm 15q
multipcf finished for chromosome arm 16p
multipcf finished for chromosome arm 16q
multipcf finished for chromosome arm 17p
multipcf finished for chromosome arm 17q
multipcf finished for chromosome arm 18p
multipcf finished for chromosome arm 18q
multipcf finished for chromosome arm 19p
multipcf finished for chromosome arm 19q
multipcf finished for chromosome arm 20p
multipcf finished for chromosome arm 20q
multipcf finished for chromosome arm 21q
multipcf finished for chromosome arm 22q
INFO [2021-10-15 04:11:02] ------------------------------------------------------------
INFO [2021-10-15 04:11:02] PureCN 1.22.2
INFO [2021-10-15 04:11:02] ------------------------------------------------------------
INFO [2021-10-15 04:11:02] Loading coverage files...
INFO [2021-10-15 04:11:03] seg.file and normal.coverage.file provided. Using both.
INFO [2021-10-15 04:11:03] Mean target coverages: 112X (tumor) 105X (normal).
WARN [2021-10-15 04:11:03] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:11:03] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:11:03] Removing 199 intervals with missing log.ratio.
INFO [2021-10-15 04:11:03] Using 9850 intervals (9850 on-target, 0 off-target).
INFO [2021-10-15 04:11:03] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:11:03] Loading VCF...
INFO [2021-10-15 04:11:04] Found 127 variants in VCF file.
INFO [2021-10-15 04:11:04] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:11:04] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:11:04] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:11:04] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:11:05] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:11:05] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:11:05] Total size of targeted genomic region: 1.68Mb (2.66Mb with 50bp padding).
INFO [2021-10-15 04:11:05] 1.0% of targets contain variants.
INFO [2021-10-15 04:11:06] Removing 0 variants outside intervals.
INFO [2021-10-15 04:11:06] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:11:06] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:11:06] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2021-10-15 04:11:07] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:11:07] Sample sex: ?
INFO [2021-10-15 04:11:07] Segmenting data...
INFO [2021-10-15 04:11:07] Loaded provided segmentation file fileabec45c12688.seg (format DNAcopy).
INFO [2021-10-15 04:11:07] Re-centering provided segment means (offset 0.0110).
INFO [2021-10-15 04:11:07] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:11:07] Found 59 segments with median size of 30.15Mb.
INFO [2021-10-15 04:11:07] Using 125 variants.
INFO [2021-10-15 04:11:07] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:11:07] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:11:09] Local optima: 0.6/1.9
INFO [2021-10-15 04:11:09] Testing local optimum 1/1 at purity 0.60 and total ploidy 1.90...
INFO [2021-10-15 04:11:11] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:11:11] Fitting variants for purity 0.60, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:15] Fitting variants for purity 0.40, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:19] Fitting variants for purity 0.45, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:22] Fitting variants for purity 0.50, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:25] Fitting variants for purity 0.55, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:29] Fitting variants for purity 0.65, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:33] Fitting variants for purity 0.70, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:11:37] Optimized purity: 0.65
INFO [2021-10-15 04:11:37] Done.
INFO [2021-10-15 04:11:37] ------------------------------------------------------------
INFO [2021-10-15 04:11:40] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
FATAL [2021-10-15 04:11:40] Cannot find centromeres for hg20. Provide them manually or select a
FATAL [2021-10-15 04:11:40] supported genome.
FATAL [2021-10-15 04:11:40]
FATAL [2021-10-15 04:11:40] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:11:40] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:11:41] Found 20 variants in VCF file.
INFO [2021-10-15 04:11:42] 20 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:11:44] Found 127 variants in VCF file.
INFO [2021-10-15 04:11:44] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:11:45] ------------------------------------------------------------
INFO [2021-10-15 04:11:45] PureCN 1.22.2
INFO [2021-10-15 04:11:45] ------------------------------------------------------------
INFO [2021-10-15 04:11:45] Loading coverage files...
INFO [2021-10-15 04:11:46] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:11:46] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:11:46] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:11:46] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:11:46] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:11:46] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:11:46] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:11:46] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:11:46] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:11:46] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:11:46] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:11:46] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:11:46] Loading VCF...
INFO [2021-10-15 04:11:46] Found 127 variants in VCF file.
INFO [2021-10-15 04:11:46] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:11:46] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:11:47] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:11:47] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:11:47] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:11:47] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:11:47] Total size of targeted genomic region: 1.53Mb (2.33Mb with 50bp padding).
INFO [2021-10-15 04:11:47] 1.2% of targets contain variants.
INFO [2021-10-15 04:11:47] Removing 4 variants outside intervals.
INFO [2021-10-15 04:11:48] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:11:48] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:11:48] Found SOMATIC annotation in VCF. Setting mapping bias to 0.972.
INFO [2021-10-15 04:11:48] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:11:48] Sample sex: ?
INFO [2021-10-15 04:11:48] Segmenting data...
INFO [2021-10-15 04:11:48] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:11:48] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:11:50] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:11:50] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:11:50] Using 121 variants.
INFO [2021-10-15 04:11:50] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:11:50] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:11:50] Local optima: 0.65/1.8, 0.52/2
INFO [2021-10-15 04:11:50] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:11:52] Testing local optimum 2/2 at purity 0.52 and total ploidy 2.00...
INFO [2021-10-15 04:11:55] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:11:56] Fitting variants with beta model for local optimum 1/2...
INFO [2021-10-15 04:11:56] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:12:01] Optimized purity: 0.65
INFO [2021-10-15 04:12:01] Done.
INFO [2021-10-15 04:12:01] ------------------------------------------------------------
FATAL [2021-10-15 04:12:24] log.ratio NULL in .writeLogRatioFileGATK4
FATAL [2021-10-15 04:12:24]
FATAL [2021-10-15 04:12:24] This runtime error might be caused by invalid input data or parameters.
FATAL [2021-10-15 04:12:24] Please report bug (PureCN 1.22.2).
INFO [2021-10-15 04:12:25] Loaded provided segmentation file example_seg.txt (format DNAcopy).
INFO [2021-10-15 04:12:25] Re-centering provided segment means (offset -0.0033).
INFO [2021-10-15 04:12:25] Loaded provided segmentation file example_gatk4_modelfinal.seg.gz (format GATK4).
WARN [2021-10-15 04:12:25] Expecting numeric chromosome names in seg.file, assuming file is properly sorted.
INFO [2021-10-15 04:12:25] Re-centering provided segment means (offset -0.0037).
INFO [2021-10-15 04:12:26] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:12:26] You are likely not using the correct baits file!
WARN [2021-10-15 04:12:26] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:12:26] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:12:26] Processing on-target regions...
INFO [2021-10-15 04:12:27] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:12:27] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:12:29] ------------------------------------------------------------
INFO [2021-10-15 04:12:29] PureCN 1.22.2
INFO [2021-10-15 04:12:29] ------------------------------------------------------------
INFO [2021-10-15 04:12:29] Using BiocParallel for parallel optimization.
INFO [2021-10-15 04:12:29] Loading coverage files...
INFO [2021-10-15 04:12:30] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:12:30] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:12:30] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:12:30] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:12:30] Removing 705 intervals excluded in normalDB.
INFO [2021-10-15 04:12:30] normalDB provided. Setting minimum coverage for segmentation to 0.0015X.
INFO [2021-10-15 04:12:30] Removing 1066 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:12:30] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:12:30] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:12:30] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:12:31] Sample sex: ?
INFO [2021-10-15 04:12:31] Segmenting data...
INFO [2021-10-15 04:12:31] Interval weights found, will use weighted CBS.
INFO [2021-10-15 04:12:31] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:12:31] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2021-10-15 04:12:33] Found 54 segments with median size of 23.50Mb.
INFO [2021-10-15 04:12:33] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:12:33] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:12:33] Local optima: 0.65/1.8, 0.52/2
INFO [2021-10-15 04:13:15] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:13:16] Done.
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmp2RVCFq\fileabec3d4253e.rds...
FATAL [2021-10-15 04:13:16] runAbsoluteCN was run without a VCF file.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:13:16] runAbsoluteCN was run without a VCF file.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] Loading coverage files...
FATAL [2021-10-15 04:13:16] Need a normal coverage file if log.ratio and seg.file are not provided.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
FATAL [2021-10-15 04:13:16] min.ploidy or max.ploidy not within expected range.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
FATAL [2021-10-15 04:13:16] min.ploidy or max.ploidy not within expected range.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
FATAL [2021-10-15 04:13:16] test.num.copy not within expected range.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
WARN [2021-10-15 04:13:16] test.num.copy outside recommended range.
FATAL [2021-10-15 04:13:16] max.non.clonal not within expected range or format.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
WARN [2021-10-15 04:13:16] test.num.copy outside recommended range.
FATAL [2021-10-15 04:13:16] max.non.clonal not within expected range or format.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
FATAL [2021-10-15 04:13:16] test.purity not within expected range.
FATAL [2021-10-15 04:13:16]
FATAL [2021-10-15 04:13:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] PureCN 1.22.2
INFO [2021-10-15 04:13:16] ------------------------------------------------------------
INFO [2021-10-15 04:13:16] Loading coverage files...
FATAL [2021-10-15 04:13:17] Tumor and normal are identical. This won't give any meaningful results
FATAL [2021-10-15 04:13:17] and I'm stopping here.
FATAL [2021-10-15 04:13:17]
FATAL [2021-10-15 04:13:17] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:17] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:17] ------------------------------------------------------------
INFO [2021-10-15 04:13:17] PureCN 1.22.2
INFO [2021-10-15 04:13:17] ------------------------------------------------------------
INFO [2021-10-15 04:13:17] Loading coverage files...
FATAL [2021-10-15 04:13:17] Length of log.ratio different from tumor coverage.
FATAL [2021-10-15 04:13:17]
FATAL [2021-10-15 04:13:17] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:17] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] prior.purity must have the same length as test.purity.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] min.gof not within expected range or format.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] prior.purity not within expected range or format.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] prior.purity must add to 1. Sum is 1.5
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] max.homozygous.loss not within expected range or format.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] prior.K not within expected range or format.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] prior.contamination not within expected range or format.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] Iterations not in the expected range from 10 to 250.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
FATAL [2021-10-15 04:13:18] Iterations not in the expected range from 10 to 250.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] Loading coverage files...
FATAL [2021-10-15 04:13:18] Missing tumor.coverage.file requires seg.file or log.ratio and
FATAL [2021-10-15 04:13:18] interval.file.
FATAL [2021-10-15 04:13:18]
FATAL [2021-10-15 04:13:18] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:18] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:18] PureCN 1.22.2
INFO [2021-10-15 04:13:18] ------------------------------------------------------------
INFO [2021-10-15 04:13:19] ------------------------------------------------------------
INFO [2021-10-15 04:13:19] PureCN 1.22.2
INFO [2021-10-15 04:13:19] ------------------------------------------------------------
INFO [2021-10-15 04:13:19] Loading coverage files...
FATAL [2021-10-15 04:13:19] Interval files in normal and tumor different.
FATAL [2021-10-15 04:13:19]
FATAL [2021-10-15 04:13:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:19] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:19] ------------------------------------------------------------
INFO [2021-10-15 04:13:19] PureCN 1.22.2
INFO [2021-10-15 04:13:19] ------------------------------------------------------------
INFO [2021-10-15 04:13:19] Loading coverage files...
INFO [2021-10-15 04:13:20] Mean target coverages: 0X (tumor) 99X (normal).
WARN [2021-10-15 04:13:20] Large difference in coverage of tumor and normal.
FATAL [2021-10-15 04:13:20] No finite intervals.
FATAL [2021-10-15 04:13:20]
FATAL [2021-10-15 04:13:20] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:20] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:20] ------------------------------------------------------------
INFO [2021-10-15 04:13:20] PureCN 1.22.2
INFO [2021-10-15 04:13:20] ------------------------------------------------------------
INFO [2021-10-15 04:13:20] Loading coverage files...
INFO [2021-10-15 04:13:21] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:13:21] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:13:21] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:13:21] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:13:21] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:13:21] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:13:21] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:13:21] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:13:21] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:13:21] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:13:21] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:13:21] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:13:21] Loading VCF...
INFO [2021-10-15 04:13:22] Found 127 variants in VCF file.
INFO [2021-10-15 04:13:22] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:13:22] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:13:22] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:13:22] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:13:22] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'con' in selecting a method for function 'import': Format 'txt' unsupported
FATAL [2021-10-15 04:13:22] Could not import snp.blacklist
FATAL [2021-10-15 04:13:22] C:/Users/biocbuild/bbs-3.13-bioc/R/library/PureCN/extdata/example_normal.txt:Error
FATAL [2021-10-15 04:13:22] in h(simpleError(msg, call)) : error in evaluating the argument 'con'
FATAL [2021-10-15 04:13:22] in selecting a method for function 'import': Format 'txt' unsupported
FATAL [2021-10-15 04:13:22]
FATAL [2021-10-15 04:13:22] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:13:22] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:13:22] ------------------------------------------------------------
INFO [2021-10-15 04:13:22] PureCN 1.22.2
INFO [2021-10-15 04:13:22] ------------------------------------------------------------
INFO [2021-10-15 04:13:22] Loading coverage files...
INFO [2021-10-15 04:13:23] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:13:23] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:13:23] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:13:23] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:13:23] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:13:23] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:13:23] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:13:23] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:13:23] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2021-10-15 04:13:23] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:13:23] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:13:23] Loading VCF...
INFO [2021-10-15 04:13:24] Found 127 variants in VCF file.
INFO [2021-10-15 04:13:24] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:13:24] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:13:24] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:13:24] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:13:25] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:13:25] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:13:25] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2021-10-15 04:13:25] 1.0% of targets contain variants.
INFO [2021-10-15 04:13:25] Removing 2 variants outside intervals.
INFO [2021-10-15 04:13:25] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:13:25] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:13:26] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2021-10-15 04:13:26] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:13:26] Sample sex: ?
INFO [2021-10-15 04:13:26] Segmenting data...
INFO [2021-10-15 04:13:26] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:13:26] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:13:28] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:13:28] Found 54 segments with median size of 24.88Mb.
INFO [2021-10-15 04:13:28] Using 123 variants.
INFO [2021-10-15 04:13:28] Mean standard deviation of log-ratios: 0.37
INFO [2021-10-15 04:13:28] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:13:30] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2021-10-15 04:13:30] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2021-10-15 04:13:32] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:32] Recalibrating log-ratios...
INFO [2021-10-15 04:13:32] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:33] Recalibrating log-ratios...
INFO [2021-10-15 04:13:33] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:33] Recalibrating log-ratios...
INFO [2021-10-15 04:13:33] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:34] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:34] Recalibrating log-ratios...
INFO [2021-10-15 04:13:34] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:35] Recalibrating log-ratios...
INFO [2021-10-15 04:13:35] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:35] Recalibrating log-ratios...
INFO [2021-10-15 04:13:35] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:36] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:13:36] Skipping 1 solutions exceeding max.non.clonal (0.20): 0.3/1.9 (purity/tumor ploidy)
INFO [2021-10-15 04:13:36] Fitting variants with beta model for local optimum 1/3...
INFO [2021-10-15 04:13:36] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2021-10-15 04:13:39] Optimized purity: 0.65
INFO [2021-10-15 04:13:39] Done.
INFO [2021-10-15 04:13:39] ------------------------------------------------------------
INFO [2021-10-15 04:13:39] ------------------------------------------------------------
INFO [2021-10-15 04:13:39] PureCN 1.22.2
INFO [2021-10-15 04:13:39] ------------------------------------------------------------
INFO [2021-10-15 04:13:39] Loading coverage files...
INFO [2021-10-15 04:13:40] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:13:40] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:13:40] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:13:40] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:13:40] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:13:40] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:13:40] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:13:40] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:13:40] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:13:40] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:13:40] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:13:40] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:13:40] Loading VCF...
INFO [2021-10-15 04:13:41] Found 127 variants in VCF file.
WARN [2021-10-15 04:13:41] DP FORMAT field contains NAs. Removing 3 variants.
WARN [2021-10-15 04:13:41] DB INFO flag contains NAs
INFO [2021-10-15 04:13:41] 119 (96.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:13:41] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:13:41] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:13:41] Removing 1 variants with AF < 0.030 or AF >= 0.970 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:13:42] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:13:42] Total size of targeted genomic region: 1.53Mb (2.33Mb with 50bp padding).
INFO [2021-10-15 04:13:42] 1.2% of targets contain variants.
INFO [2021-10-15 04:13:42] Setting somatic prior probabilities for dbSNP hits to 0.000500 or to 0.500000 otherwise.
INFO [2021-10-15 04:13:42] Excluding 5 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:13:42] Sample sex: ?
INFO [2021-10-15 04:13:42] Segmenting data...
INFO [2021-10-15 04:13:43] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:13:43] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2021-10-15 04:13:45] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:13:45] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:13:45] Using 123 variants.
INFO [2021-10-15 04:13:45] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:13:45] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:13:47] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2021-10-15 04:13:47] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2021-10-15 04:13:49] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:49] Recalibrating log-ratios...
INFO [2021-10-15 04:13:49] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:50] Recalibrating log-ratios...
INFO [2021-10-15 04:13:50] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:50] Recalibrating log-ratios...
INFO [2021-10-15 04:13:50] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:13:50] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:51] Recalibrating log-ratios...
INFO [2021-10-15 04:13:51] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:51] Recalibrating log-ratios...
INFO [2021-10-15 04:13:51] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:52] Recalibrating log-ratios...
INFO [2021-10-15 04:13:52] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:13:52] Skipping 2 solutions exceeding max.non.clonal (0.20): 0.3/1.9, 0.35/1.67 (purity/tumor ploidy)
INFO [2021-10-15 04:13:52] Fitting variants with beta model for local optimum 1/3...
INFO [2021-10-15 04:13:52] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:13:56] Optimized purity: 0.65
INFO [2021-10-15 04:13:56] Done.
INFO [2021-10-15 04:13:56] ------------------------------------------------------------
INFO [2021-10-15 04:13:56] ------------------------------------------------------------
INFO [2021-10-15 04:13:56] PureCN 1.22.2
INFO [2021-10-15 04:13:56] ------------------------------------------------------------
INFO [2021-10-15 04:13:56] Loading coverage files...
INFO [2021-10-15 04:13:57] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:13:57] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:13:57] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:13:57] No Gene column in interval.file. You won't get gene-level calls.
INFO [2021-10-15 04:13:58] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:13:59] Removing 15 low/high GC targets.
INFO [2021-10-15 04:13:59] Removing 21 small (< 5bp) intervals.
INFO [2021-10-15 04:13:59] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:13:59] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:13:59] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:13:59] Removing 1492 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:13:59] Removing 36 low mappability intervals.
INFO [2021-10-15 04:13:59] Using 8006 intervals (8006 on-target, 0 off-target).
INFO [2021-10-15 04:13:59] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:13:59] AT/GC dropout: 1.01 (tumor), 1.03 (normal), 0.98 (coverage log-ratio).
INFO [2021-10-15 04:13:59] Loading VCF...
INFO [2021-10-15 04:13:59] Found 127 variants in VCF file.
INFO [2021-10-15 04:13:59] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:14:00] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:14:00] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:14:00] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:14:00] Removing 1 variants with AF < 0.030 or AF >= Inf or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:14:00] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:14:00] Total size of targeted genomic region: 1.51Mb (2.31Mb with 50bp padding).
INFO [2021-10-15 04:14:01] 1.2% of targets contain variants.
INFO [2021-10-15 04:14:01] Removing 11 variants outside intervals.
INFO [2021-10-15 04:14:01] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:14:01] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:14:01] Found SOMATIC annotation in VCF. Setting mapping bias to 0.970.
INFO [2021-10-15 04:14:01] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:14:01] Sample sex: ?
INFO [2021-10-15 04:14:01] Segmenting data...
INFO [2021-10-15 04:14:02] Using unweighted PSCBS.
INFO [2021-10-15 04:14:02] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:14:17] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:14:18] Found 72 segments with median size of 27.25Mb.
INFO [2021-10-15 04:14:18] Using 114 variants.
INFO [2021-10-15 04:14:18] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:14:18] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:14:18] Local optima: 0.65/1.8, 0.38/2.2
INFO [2021-10-15 04:14:18] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:14:18] Recalibrating log-ratios...
INFO [2021-10-15 04:14:18] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:14:18] Recalibrating log-ratios...
INFO [2021-10-15 04:14:18] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:14:19] Recalibrating log-ratios...
INFO [2021-10-15 04:14:19] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:14:19] Testing local optimum 2/2 at purity 0.38 and total ploidy 2.20...
INFO [2021-10-15 04:14:20] Skipping 1 solutions exceeding max.non.clonal (0.20): 0.65/2.06 (purity/tumor ploidy)
INFO [2021-10-15 04:14:20] Fitting variants with beta model for local optimum 2/2...
INFO [2021-10-15 04:14:20] Fitting variants for purity 0.40, tumor ploidy 2.55 and contamination 0.01.
INFO [2021-10-15 04:14:23] Optimized purity: 0.40
INFO [2021-10-15 04:14:23] Done.
INFO [2021-10-15 04:14:23] ------------------------------------------------------------
FATAL [2021-10-15 04:14:23] This function requires gene-level calls. Please add a column 'Gene'
FATAL [2021-10-15 04:14:23] containing gene symbols to the interval.file.
FATAL [2021-10-15 04:14:23]
FATAL [2021-10-15 04:14:23] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:14:23] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:14:24] ------------------------------------------------------------
INFO [2021-10-15 04:14:24] PureCN 1.22.2
INFO [2021-10-15 04:14:24] ------------------------------------------------------------
INFO [2021-10-15 04:14:24] Loading coverage files...
INFO [2021-10-15 04:14:25] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:14:25] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:14:25] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:14:25] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:14:25] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:14:25] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:14:25] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:14:25] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:14:25] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:14:25] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:14:25] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:14:25] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:14:25] Loading VCF...
INFO [2021-10-15 04:14:25] Found 127 variants in VCF file.
INFO [2021-10-15 04:14:25] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
FATAL [2021-10-15 04:14:26] Different chromosome names in coverage and VCF.
FATAL [2021-10-15 04:14:26]
FATAL [2021-10-15 04:14:26] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:14:26] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:14:26] ------------------------------------------------------------
INFO [2021-10-15 04:14:26] PureCN 1.22.2
INFO [2021-10-15 04:14:26] ------------------------------------------------------------
INFO [2021-10-15 04:14:26] Loading coverage files...
INFO [2021-10-15 04:14:26] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:14:26] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:14:26] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:14:26] tumor.coverage.file and interval.file do not align.
FATAL [2021-10-15 04:14:26]
FATAL [2021-10-15 04:14:26] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:14:26] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:14:27] ------------------------------------------------------------
INFO [2021-10-15 04:14:27] PureCN 1.22.2
INFO [2021-10-15 04:14:27] ------------------------------------------------------------
INFO [2021-10-15 04:14:27] Loading coverage files...
INFO [2021-10-15 04:14:27] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:14:27] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:14:27] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:14:28] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:14:28] Removing 15 low/high GC targets.
INFO [2021-10-15 04:14:28] Removing 21 small (< 5bp) intervals.
INFO [2021-10-15 04:14:28] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:14:28] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:14:28] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:14:28] Removing 1492 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:14:28] Removing 36 low mappability intervals.
INFO [2021-10-15 04:14:28] Using 8006 intervals (8006 on-target, 0 off-target).
INFO [2021-10-15 04:14:28] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:14:28] AT/GC dropout: 1.01 (tumor), 1.03 (normal), 0.98 (coverage log-ratio).
INFO [2021-10-15 04:14:28] Loading VCF...
INFO [2021-10-15 04:14:28] Found 127 variants in VCF file.
INFO [2021-10-15 04:14:28] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:14:28] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:14:29] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:14:29] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:14:29] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:14:29] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:14:29] Total size of targeted genomic region: 1.51Mb (2.31Mb with 50bp padding).
INFO [2021-10-15 04:14:29] 1.2% of targets contain variants.
INFO [2021-10-15 04:14:29] Removing 11 variants outside intervals.
INFO [2021-10-15 04:14:29] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:14:29] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:14:30] Found SOMATIC annotation in VCF. Setting mapping bias to 0.970.
INFO [2021-10-15 04:14:30] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:14:30] Sample sex: ?
INFO [2021-10-15 04:14:30] Segmenting data...
INFO [2021-10-15 04:14:30] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:14:30] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:14:31] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:14:31] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:14:31] Using 114 variants.
INFO [2021-10-15 04:14:31] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:14:31] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:14:32] Local optima: 0.63/1.9
INFO [2021-10-15 04:14:32] Testing local optimum 1/1 at purity 0.63 and total ploidy 1.90...
INFO [2021-10-15 04:14:33] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:14:33] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:14:36] Optimized purity: 0.65
INFO [2021-10-15 04:14:36] Done.
INFO [2021-10-15 04:14:36] ------------------------------------------------------------
INFO [2021-10-15 04:14:36] ------------------------------------------------------------
INFO [2021-10-15 04:14:36] PureCN 1.22.2
INFO [2021-10-15 04:14:36] ------------------------------------------------------------
INFO [2021-10-15 04:14:36] Loading coverage files...
INFO [2021-10-15 04:14:36] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:14:36] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:14:36] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:14:37] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:14:37] Removing 15 low/high GC targets.
INFO [2021-10-15 04:14:38] Removing 21 small (< 5bp) intervals.
INFO [2021-10-15 04:14:38] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:14:38] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:14:38] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:14:38] Removing 1492 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:14:38] Removing 36 low mappability intervals.
INFO [2021-10-15 04:14:38] Using 8006 intervals (8006 on-target, 0 off-target).
INFO [2021-10-15 04:14:38] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:14:38] AT/GC dropout: 1.01 (tumor), 1.03 (normal), 0.98 (coverage log-ratio).
INFO [2021-10-15 04:14:38] Loading VCF...
INFO [2021-10-15 04:14:38] Found 127 variants in VCF file.
INFO [2021-10-15 04:14:38] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:14:38] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:14:38] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:14:38] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:14:39] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:14:39] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:14:39] Total size of targeted genomic region: 1.51Mb (2.31Mb with 50bp padding).
INFO [2021-10-15 04:14:39] 1.2% of targets contain variants.
INFO [2021-10-15 04:14:39] Removing 11 variants outside intervals.
INFO [2021-10-15 04:14:39] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:14:39] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:14:39] Found SOMATIC annotation in VCF. Setting mapping bias to 0.970.
INFO [2021-10-15 04:14:40] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:14:40] Sample sex: ?
INFO [2021-10-15 04:14:40] Segmenting data...
INFO [2021-10-15 04:14:40] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:14:40] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:14:41] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:14:41] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:14:41] Using 114 variants.
INFO [2021-10-15 04:14:41] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:14:41] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:14:42] Local optima: 0.63/1.9
INFO [2021-10-15 04:14:42] Testing local optimum 1/1 at purity 0.63 and total ploidy 1.90...
INFO [2021-10-15 04:14:43] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:14:43] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:14:46] Optimized purity: 0.65
INFO [2021-10-15 04:14:46] Done.
INFO [2021-10-15 04:14:46] ------------------------------------------------------------
FATAL [2021-10-15 04:14:46] chr1 not valid chromosome name(s). Valid names are:
FATAL [2021-10-15 04:14:46] 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22
FATAL [2021-10-15 04:14:46]
FATAL [2021-10-15 04:14:46] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:14:46] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:14:46] ------------------------------------------------------------
INFO [2021-10-15 04:14:46] PureCN 1.22.2
INFO [2021-10-15 04:14:46] ------------------------------------------------------------
INFO [2021-10-15 04:14:46] Loading coverage files...
WARN [2021-10-15 04:14:46] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
WARN [2021-10-15 04:14:46] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:14:46] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:14:46] Removing 10 intervals with missing log.ratio.
INFO [2021-10-15 04:14:46] Using 10039 intervals (10039 on-target, 0 off-target).
INFO [2021-10-15 04:14:46] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:14:46] Loading VCF...
INFO [2021-10-15 04:14:47] Found 127 variants in VCF file.
INFO [2021-10-15 04:14:47] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:14:47] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:14:47] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:14:47] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:14:47] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:14:47] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:14:48] Total size of targeted genomic region: 1.71Mb (2.70Mb with 50bp padding).
INFO [2021-10-15 04:14:48] 1.0% of targets contain variants.
INFO [2021-10-15 04:14:48] Removing 0 variants outside intervals.
INFO [2021-10-15 04:14:48] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:14:48] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:14:48] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2021-10-15 04:14:48] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:14:48] Sample sex: ?
INFO [2021-10-15 04:14:48] Segmenting data...
INFO [2021-10-15 04:14:48] Loaded provided segmentation file example_seg.txt (format DNAcopy).
WARN [2021-10-15 04:14:48] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
INFO [2021-10-15 04:14:48] Re-centering provided segment means (offset -0.0033).
INFO [2021-10-15 04:14:48] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:14:48] Setting undo.SD parameter to 0.000000.
Setting multi-figure configuration
INFO [2021-10-15 04:14:49] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:14:49] Found 54 segments with median size of 24.88Mb.
INFO [2021-10-15 04:14:49] Using 125 variants.
INFO [2021-10-15 04:14:49] Mean standard deviation of log-ratios: 0.40
INFO [2021-10-15 04:14:49] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:14:50] Local optima: 0.6/1.9
INFO [2021-10-15 04:14:50] Testing local optimum 1/1 at purity 0.60 and total ploidy 1.90...
INFO [2021-10-15 04:14:51] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:14:51] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2021-10-15 04:14:53] Optimized purity: 0.65
INFO [2021-10-15 04:14:53] Done.
INFO [2021-10-15 04:14:53] ------------------------------------------------------------
WARN [2021-10-15 04:14:54] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:14:54] Allosome coverage missing, cannot determine sex.
Setting multi-figure configuration
FATAL [2021-10-15 04:15:02] Segmentation file expected with colnames ID, chrom, loc.start, loc.end,
FATAL [2021-10-15 04:15:02] num.mark, seg.mean
FATAL [2021-10-15 04:15:02]
FATAL [2021-10-15 04:15:02] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:15:02] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:15:03] seg.file contains multiple samples and sampleid missing.
FATAL [2021-10-15 04:15:03]
FATAL [2021-10-15 04:15:03] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:15:03] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:15:03] seg.file contains multiple samples and sampleid does not match any.
FATAL [2021-10-15 04:15:03]
FATAL [2021-10-15 04:15:03] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:15:03] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:15:03] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:03] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:13] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:13] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:13] Provided sampleid (example_tumor.txt) does not match Sample1 found in segmentation.
WARN [2021-10-15 04:15:16] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:16] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:18] No normalDB provided. Provide one for better results.
WARN [2021-10-15 04:15:27] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:27] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:30] Provided sampleid (Sample.1) does not match Sample1 found in segmentation.
Setting multi-figure configuration
WARN [2021-10-15 04:15:40] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:40] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:40] No normalDB provided. Provide one for better results.
WARN [2021-10-15 04:15:40] Sampleid looks like a normal in VCF, not like a tumor.
WARN [2021-10-15 04:15:55] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:55] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:15:56] normalDB not a valid normalDB object. Use createNormalDatabase to
FATAL [2021-10-15 04:15:56] create one.
FATAL [2021-10-15 04:15:56]
FATAL [2021-10-15 04:15:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:15:56] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:15:56] You are likely not using the correct baits file!
WARN [2021-10-15 04:15:56] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:56] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:59] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:15:59] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:15:59] normalDB appears to be empty.
FATAL [2021-10-15 04:15:59]
FATAL [2021-10-15 04:15:59] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:15:59] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:16:00] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:16:00] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:16:01] Intervals in coverage and interval.file have conflicting on/off-target annotation.
WARN [2021-10-15 04:16:15] Cannot find gatk binary in path.
FATAL [2021-10-15 04:17:00] The normal.panel.vcf.file contains only a single sample.
FATAL [2021-10-15 04:17:00]
FATAL [2021-10-15 04:17:00] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:00] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:00] mapping.bias.file must be a file with *.rds suffix.
FATAL [2021-10-15 04:17:00]
FATAL [2021-10-15 04:17:00] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:00] parameters (PureCN 1.22.2).
Failed with error: 'there is no package called 'genomicsdb''
== Skipped tests ===============================================================
* gatk binary > 4.1.7.0 required (1)
* genomicsdb required (1)
[ FAIL 0 | WARN 53 | SKIP 2 | PASS 367 ]
>
> proc.time()
user system elapsed
515.32 6.70 669.93
|
PureCN.Rcheck/tests_x64/testthat.Rout
R version 4.1.1 (2021-08-10) -- "Kick Things"
Copyright (C) 2021 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(PureCN)
Loading required package: DNAcopy
Loading required package: VariantAnnotation
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
tapply, union, unique, unsplit, which.max, which.min
Loading required package: MatrixGenerics
Loading required package: matrixStats
Attaching package: 'MatrixGenerics'
The following objects are masked from 'package:matrixStats':
colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
colWeightedMeans, colWeightedMedians, colWeightedSds,
colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
rowWeightedSds, rowWeightedVars
Loading required package: GenomeInfoDb
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Attaching package: 'IRanges'
The following object is masked from 'package:grDevices':
windows
Loading required package: GenomicRanges
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Attaching package: 'Biobase'
The following object is masked from 'package:MatrixGenerics':
rowMedians
The following objects are masked from 'package:matrixStats':
anyMissing, rowMedians
Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
Attaching package: 'VariantAnnotation'
The following object is masked from 'package:base':
tabulate
>
> test_check("PureCN")
WARN [2021-10-15 04:17:22] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
WARN [2021-10-15 04:17:22] Chromosome naming style of txdb file (UCSC) was different from interval file (Ensembl).
WARN [2021-10-15 04:17:25] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
FATAL [2021-10-15 04:17:27] tumor.coverage.file and interval.file do not align.
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:27] Need either f or purity and ploidy.
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:27] f not in expected range.
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:27] coverage not in expected range (>=2)
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:27] purity not in expected range.
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:27] ploidy not in expected range.
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:17:27] cell.fraction not in expected range.
FATAL [2021-10-15 04:17:27]
FATAL [2021-10-15 04:17:27] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:17:27] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:17:30] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:17:30] You are likely not using the correct baits file!
WARN [2021-10-15 04:17:30] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:17:30] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:17:30] Processing on-target regions...
INFO [2021-10-15 04:17:30] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:17:30] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:17:33] Tumor/normal noise ratio: 19.041
WARN [2021-10-15 04:17:33] Extensive noise in tumor compared to normals.
INFO [2021-10-15 04:18:37] Tumor/normal noise ratio: 19.041
WARN [2021-10-15 04:18:37] Extensive noise in tumor compared to normals.
INFO [2021-10-15 04:18:38] Using BiocParallel for parallel optimization.
FATAL [2021-10-15 04:19:53] pvalue.cutoff not within expected range or format.
FATAL [2021-10-15 04:19:53]
FATAL [2021-10-15 04:19:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:19:53] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:19:53] pvalue.cutoff not within expected range or format.
FATAL [2021-10-15 04:19:53]
FATAL [2021-10-15 04:19:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:19:53] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:19:53] percentile.cutoff not in expected range (0 to 100).
FATAL [2021-10-15 04:19:53]
FATAL [2021-10-15 04:19:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:19:53] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:19:53] percentile.cutoff not in expected range (0 to 100).
FATAL [2021-10-15 04:19:53]
FATAL [2021-10-15 04:19:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:19:53] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:19:53] purity not within expected range or format.
FATAL [2021-10-15 04:19:53]
FATAL [2021-10-15 04:19:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:19:53] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:19:53] purity not within expected range or format.
FATAL [2021-10-15 04:19:53]
FATAL [2021-10-15 04:19:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:19:53] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:19:56] ------------------------------------------------------------
INFO [2021-10-15 04:19:56] PureCN 1.22.2
INFO [2021-10-15 04:19:56] ------------------------------------------------------------
INFO [2021-10-15 04:19:56] Loading coverage files...
INFO [2021-10-15 04:19:56] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:19:56] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:19:56] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:19:56] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:19:56] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:19:56] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:19:56] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:19:56] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:19:56] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:19:56] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:19:56] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:19:56] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:19:56] Loading VCF...
INFO [2021-10-15 04:19:56] Found 127 variants in VCF file.
INFO [2021-10-15 04:19:56] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:19:57] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:19:57] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:19:57] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:19:57] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:19:57] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:19:58] Total size of targeted genomic region: 1.53Mb (2.33Mb with 50bp padding).
INFO [2021-10-15 04:19:58] 1.2% of targets contain variants.
INFO [2021-10-15 04:19:58] Removing 4 variants outside intervals.
INFO [2021-10-15 04:19:58] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:19:58] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:19:59] Found SOMATIC annotation in VCF. Setting mapping bias to 0.972.
INFO [2021-10-15 04:19:59] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:19:59] Sample sex: ?
INFO [2021-10-15 04:19:59] Segmenting data...
INFO [2021-10-15 04:19:59] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:19:59] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:20:00] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:20:01] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:20:01] Using 121 variants.
INFO [2021-10-15 04:20:01] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:20:01] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:20:02] Local optima: 0.63/1.9, 0.5/2
INFO [2021-10-15 04:20:02] Testing local optimum 1/2 at purity 0.63 and total ploidy 1.90...
INFO [2021-10-15 04:20:03] Testing local optimum 2/2 at purity 0.50 and total ploidy 2.00...
INFO [2021-10-15 04:20:04] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:20:04] Fitting variants with beta model for local optimum 1/2...
INFO [2021-10-15 04:20:04] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:20:07] Optimized purity: 0.65
INFO [2021-10-15 04:20:07] Done.
INFO [2021-10-15 04:20:07] ------------------------------------------------------------
INFO [2021-10-15 04:20:08] Estimating callable regions.
FATAL [2021-10-15 04:20:10] exclude not a GRanges object.
FATAL [2021-10-15 04:20:10]
FATAL [2021-10-15 04:20:10] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:10] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:20:10] callable not a GRanges object.
FATAL [2021-10-15 04:20:10]
FATAL [2021-10-15 04:20:10] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:10] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:20:22] tumor.coverage.file and interval.file do not align.
FATAL [2021-10-15 04:20:25] No gc_bias column in interval.file.
FATAL [2021-10-15 04:20:25]
FATAL [2021-10-15 04:20:25] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:25] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:20:26] Provided coverage is zero, most likely due to a corrupt BAM file.
FATAL [2021-10-15 04:20:26]
FATAL [2021-10-15 04:20:26] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:26] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:20:32] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
INFO [2021-10-15 04:20:32] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
INFO [2021-10-15 04:20:32] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
INFO [2021-10-15 04:20:33] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
INFO [2021-10-15 04:20:33] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
INFO [2021-10-15 04:20:33] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
FATAL [2021-10-15 04:20:33] Purity or Ploidy not numeric or in expected range.
FATAL [2021-10-15 04:20:33]
FATAL [2021-10-15 04:20:33] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:33] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:20:33] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
INFO [2021-10-15 04:20:33] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.rds...
FATAL [2021-10-15 04:20:33] 'Failed' column in
FATAL [2021-10-15 04:20:33] C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf8292173d5.csv
FATAL [2021-10-15 04:20:33] not logical(1).
FATAL [2021-10-15 04:20:33]
FATAL [2021-10-15 04:20:33] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:33] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:20:34] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:20:34] You are likely not using the correct baits file!
WARN [2021-10-15 04:20:34] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:34] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:34] Processing on-target regions...
INFO [2021-10-15 04:20:35] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:20:35] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:20:39] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:20:39] You are likely not using the correct baits file!
WARN [2021-10-15 04:20:39] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:39] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:39] Processing on-target regions...
INFO [2021-10-15 04:20:39] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:20:39] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:20:42] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:20:42] You are likely not using the correct baits file!
WARN [2021-10-15 04:20:42] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:42] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:42] Processing on-target regions...
INFO [2021-10-15 04:20:43] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:20:43] Removing 1 intervals with zero coverage in more than 3% of normalDB.
WARN [2021-10-15 04:20:44] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:44] Sample sex: NA
WARN [2021-10-15 04:20:44] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:44] Sample sex: NA
INFO [2021-10-15 04:20:46] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:20:46] You are likely not using the correct baits file!
WARN [2021-10-15 04:20:46] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:46] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:20:46] Length of normal.coverage.files and sex different
FATAL [2021-10-15 04:20:46]
FATAL [2021-10-15 04:20:46] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:46] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:20:51] Target intervals were not sorted.
INFO [2021-10-15 04:20:51] 560 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:20:51] You are likely not using the correct baits file!
WARN [2021-10-15 04:20:51] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:51] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:51] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:51] Processing on-target regions...
INFO [2021-10-15 04:20:52] Removing 978 intervals with low coverage in normalDB.
INFO [2021-10-15 04:20:52] Removing 11 intervals with zero coverage in more than 3% of normalDB.
FATAL [2021-10-15 04:20:55] tumor.coverage.file and normalDB do not align.
FATAL [2021-10-15 04:20:55]
FATAL [2021-10-15 04:20:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:55] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:20:55] At least 2 normal.coverage.files required.
FATAL [2021-10-15 04:20:55]
FATAL [2021-10-15 04:20:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:20:59] ------------------------------------------------------------
INFO [2021-10-15 04:20:59] PureCN 1.22.2
INFO [2021-10-15 04:20:59] ------------------------------------------------------------
INFO [2021-10-15 04:20:59] Loading coverage files...
INFO [2021-10-15 04:20:59] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:20:59] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:20:59] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:20:59] Removing 228 intervals with missing log.ratio.
FATAL [2021-10-15 04:20:59] normalDB incompatible with this PureCN version. Please re-run
FATAL [2021-10-15 04:20:59] NormalDB.R.
FATAL [2021-10-15 04:20:59]
FATAL [2021-10-15 04:20:59] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:20:59] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:21:00] normalDB incompatible with this PureCN version. Please re-run
FATAL [2021-10-15 04:21:00] NormalDB.R.
FATAL [2021-10-15 04:21:00]
FATAL [2021-10-15 04:21:00] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:00] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:21:00] normal.coverage.files with _coverage.txt and _loess.txt suffix
FATAL [2021-10-15 04:21:00] provided. Provide either only GC-normalized or raw coverage files!
FATAL [2021-10-15 04:21:00]
FATAL [2021-10-15 04:21:00] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:00] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:21:00] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:00] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:00] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:00] Removing 16 low quality variants with BQ < 25.
INFO [2021-10-15 04:21:01] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:01] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:01] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:01] Removing 6 blacklisted variants.
INFO [2021-10-15 04:21:01] Removing 16 low quality variants with BQ < 25.
INFO [2021-10-15 04:21:01] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:02] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:02] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:02] Removing 16 low quality variants with BQ < 25.
WARN [2021-10-15 04:21:02] MuTect stats file lacks contig and position columns.
INFO [2021-10-15 04:21:02] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:02] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:02] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:02] Removing 16 low quality variants with BQ < 25.
WARN [2021-10-15 04:21:03] MuTect stats file and VCF file do not align perfectly. Will remove 2325 unmatched variants.
WARN [2021-10-15 04:21:03] MuTect stats file lacks failure_reasons column. Keeping all variants listed in stats file.
INFO [2021-10-15 04:21:03] Removing 0 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:03] Removing 0 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:03] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:21:03] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:03] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:03] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:04] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:04] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:04] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:04] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:04] Initial testing for significant sample cross-contamination: unlikely
INFO [2021-10-15 04:21:05] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
FATAL [2021-10-15 04:21:05] No variants passed filter BQ.
FATAL [2021-10-15 04:21:05]
FATAL [2021-10-15 04:21:05] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:05] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:21:05] Found 11 variants in VCF file.
WARN [2021-10-15 04:21:05] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2021-10-15 04:21:05] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:21:08] Found 11 variants in VCF file.
WARN [2021-10-15 04:21:08] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2021-10-15 04:21:08] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:21:09] Found 11 variants in VCF file.
WARN [2021-10-15 04:21:09] vcf.file has no DB or POP_AF info field for membership in germline databases. Guessing it based on available dbSNP ID.
INFO [2021-10-15 04:21:10] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:21:10] Found 1000 variants in VCF file.
INFO [2021-10-15 04:21:10] Removing 2 triallelic sites.
WARN [2021-10-15 04:21:11] Having trouble guessing SOMATIC status...
WARN [2021-10-15 04:21:11] DP FORMAT field contains NAs. Removing 44 variants.
INFO [2021-10-15 04:21:11] 954 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:21:13] Found 12 variants in VCF file.
INFO [2021-10-15 04:21:13] Removing 1 triallelic sites.
WARN [2021-10-15 04:21:13] DP FORMAT field contains NAs. Removing 1 variants.
WARN [2021-10-15 04:21:13] vcf.file has no DB info field for membership in germline databases. Found and used somatic status instead.
INFO [2021-10-15 04:21:14] 8 (80.0%) variants annotated as likely germline (DB INFO flag).
WARN [2021-10-15 04:21:14] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:21:15] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:21:15] Mean coverages: chrX: 108.58, chrY: 108.58, chr1-22: 104.87.
INFO [2021-10-15 04:21:15] Mean coverages: chrX: 108.58, chrY: 2.17, chr1-22: 104.87.
INFO [2021-10-15 04:21:15] Mean coverages: chrX: 108.58, chrY: 5.17, chr1-22: 104.87.
INFO [2021-10-15 04:21:15] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:21:15] No germline variants in VCF.
FATAL [2021-10-15 04:21:16] No solution with id hello
FATAL [2021-10-15 04:21:16]
FATAL [2021-10-15 04:21:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:16] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:21:16] No solution with id 100
FATAL [2021-10-15 04:21:16]
FATAL [2021-10-15 04:21:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:16] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:21:17] all.data and w have different lengths.
FATAL [2021-10-15 04:21:17]
FATAL [2021-10-15 04:21:17] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:17] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:21:18] No mappability scores provided.
WARN [2021-10-15 04:21:18] No reptiming scores provided.
INFO [2021-10-15 04:21:18] Calculating GC-content...
WARN [2021-10-15 04:21:18] No mappability scores provided.
WARN [2021-10-15 04:21:18] No reptiming scores provided.
INFO [2021-10-15 04:21:18] Calculating GC-content...
INFO [2021-10-15 04:21:19] Removing 1 targets overlapping with exclude.
WARN [2021-10-15 04:21:19] No mappability scores provided.
WARN [2021-10-15 04:21:19] No reptiming scores provided.
INFO [2021-10-15 04:21:19] Calculating GC-content...
WARN [2021-10-15 04:21:19] Found small target regions (< 100bp). Will resize them.
FATAL [2021-10-15 04:21:19] off.target.padding must be negative.
FATAL [2021-10-15 04:21:19]
FATAL [2021-10-15 04:21:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:19] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:21:19] Interval coordinates should start at 1, not at 0
FATAL [2021-10-15 04:21:19]
FATAL [2021-10-15 04:21:19] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:19] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:21:19] Found small target regions (< 100bp). Will resize them.
FATAL [2021-10-15 04:21:20] No off-target regions after filtering for mappability and
FATAL [2021-10-15 04:21:20] off.target.padding
FATAL [2021-10-15 04:21:20]
FATAL [2021-10-15 04:21:20] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:20] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:21:20] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:21:21] No mappability scores provided.
INFO [2021-10-15 04:21:21] Calculating GC-content...
WARN [2021-10-15 04:21:21] Found small target regions (< 100bp). Will resize them.
INFO [2021-10-15 04:21:21] Averaging reptiming into bins of size 200...
WARN [2021-10-15 04:21:21] No mappability scores provided.
INFO [2021-10-15 04:21:21] Calculating GC-content...
WARN [2021-10-15 04:21:22] Found small target regions (< 100bp). Will resize them.
INFO [2021-10-15 04:21:22] Splitting 1 large targets to an average width of 200.
WARN [2021-10-15 04:21:22] No mappability scores provided.
WARN [2021-10-15 04:21:22] No reptiming scores provided.
INFO [2021-10-15 04:21:22] Calculating GC-content...
WARN [2021-10-15 04:21:22] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:21:22] No mappability scores provided.
WARN [2021-10-15 04:21:22] No reptiming scores provided.
INFO [2021-10-15 04:21:22] Calculating GC-content...
INFO [2021-10-15 04:21:23] Tiling off-target regions to an average width of 200000.
WARN [2021-10-15 04:21:23] No mappability scores provided.
WARN [2021-10-15 04:21:23] No reptiming scores provided.
INFO [2021-10-15 04:21:23] Calculating GC-content...
WARN [2021-10-15 04:21:23] Intervals contain off-target regions. Will not change intervals.
WARN [2021-10-15 04:21:23] No mappability scores provided.
WARN [2021-10-15 04:21:23] No reptiming scores provided.
INFO [2021-10-15 04:21:23] Calculating GC-content...
WARN [2021-10-15 04:21:24] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:21:24] No reptiming scores provided.
INFO [2021-10-15 04:21:24] Calculating GC-content...
WARN [2021-10-15 04:21:25] Found small target regions (< 100bp). Will resize them.
WARN [2021-10-15 04:21:25] 1 intervals without mappability score (1 on-target).
INFO [2021-10-15 04:21:26] Removing 1 intervals with low mappability score (<0.60).
WARN [2021-10-15 04:21:26] No reptiming scores provided.
INFO [2021-10-15 04:21:26] Calculating GC-content...
WARN [2021-10-15 04:21:26] Found small target regions (< 100bp). Will resize them.
INFO [2021-10-15 04:21:26] Tiling off-target regions to an average width of 200000.
WARN [2021-10-15 04:21:27] No reptiming scores provided.
INFO [2021-10-15 04:21:27] Calculating GC-content...
WARN [2021-10-15 04:21:27] No mappability scores provided.
WARN [2021-10-15 04:21:27] No reptiming scores provided.
INFO [2021-10-15 04:21:27] Calculating GC-content...
WARN [2021-10-15 04:21:28] Chromosome naming style of interval file (Ensembl) was different from reference (UCSC).
WARN [2021-10-15 04:21:28] No mappability scores provided.
WARN [2021-10-15 04:21:28] No reptiming scores provided.
INFO [2021-10-15 04:21:28] Calculating GC-content...
FATAL [2021-10-15 04:21:28] Chromosome naming style of interval file unknown, should be UCSC.
FATAL [2021-10-15 04:21:28]
FATAL [2021-10-15 04:21:28] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:21:28] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:21:28] Chromosome naming style of interval file (Ensembl) was different from reference (UCSC).
WARN [2021-10-15 04:21:28] Chromosome naming style of mappability file (Ensembl) was different from reference (UCSC).
WARN [2021-10-15 04:21:29] No reptiming scores provided.
INFO [2021-10-15 04:21:29] Calculating GC-content...
WARN [2021-10-15 04:21:29] Found small target regions (< 60bp). Will resize them.
WARN [2021-10-15 04:21:29] No mappability scores provided.
WARN [2021-10-15 04:21:29] No reptiming scores provided.
INFO [2021-10-15 04:21:29] Calculating GC-content...
WARN [2021-10-15 04:21:29] Found small target regions (< 60bp). Will drop them.
WARN [2021-10-15 04:21:30] No mappability scores provided.
WARN [2021-10-15 04:21:30] No reptiming scores provided.
INFO [2021-10-15 04:21:30] Calculating GC-content...
WARN [2021-10-15 04:21:30] Found small target regions (< 200bp). Will resize them.
WARN [2021-10-15 04:21:30] No mappability scores provided.
WARN [2021-10-15 04:21:30] No reptiming scores provided.
INFO [2021-10-15 04:21:30] Calculating GC-content...
INFO [2021-10-15 04:21:31] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:21:31] You are likely not using the correct baits file!
WARN [2021-10-15 04:21:31] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:21:31] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:21:31] Processing on-target regions...
INFO [2021-10-15 04:21:31] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:21:31] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:21:36] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
multipcf finished for chromosome arm 1p
multipcf finished for chromosome arm 1q
multipcf finished for chromosome arm 2p
multipcf finished for chromosome arm 2q
multipcf finished for chromosome arm 3p
multipcf finished for chromosome arm 3q
multipcf finished for chromosome arm 4p
multipcf finished for chromosome arm 4q
multipcf finished for chromosome arm 5p
multipcf finished for chromosome arm 5q
multipcf finished for chromosome arm 6p
multipcf finished for chromosome arm 6q
multipcf finished for chromosome arm 7p
multipcf finished for chromosome arm 7q
multipcf finished for chromosome arm 8p
multipcf finished for chromosome arm 8q
multipcf finished for chromosome arm 9p
multipcf finished for chromosome arm 9q
multipcf finished for chromosome arm 10p
multipcf finished for chromosome arm 10q
multipcf finished for chromosome arm 11p
multipcf finished for chromosome arm 11q
multipcf finished for chromosome arm 12p
multipcf finished for chromosome arm 12q
multipcf finished for chromosome arm 13q
multipcf finished for chromosome arm 14q
multipcf finished for chromosome arm 15q
multipcf finished for chromosome arm 16p
multipcf finished for chromosome arm 16q
multipcf finished for chromosome arm 17p
multipcf finished for chromosome arm 17q
multipcf finished for chromosome arm 18p
multipcf finished for chromosome arm 18q
multipcf finished for chromosome arm 19p
multipcf finished for chromosome arm 19q
multipcf finished for chromosome arm 20p
multipcf finished for chromosome arm 20q
multipcf finished for chromosome arm 21q
multipcf finished for chromosome arm 22q
INFO [2021-10-15 04:21:39] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
multipcf finished for chromosome arm 1p
multipcf finished for chromosome arm 1q
multipcf finished for chromosome arm 2p
multipcf finished for chromosome arm 2q
multipcf finished for chromosome arm 3p
multipcf finished for chromosome arm 3q
multipcf finished for chromosome arm 4p
multipcf finished for chromosome arm 4q
multipcf finished for chromosome arm 5p
multipcf finished for chromosome arm 5q
multipcf finished for chromosome arm 6p
multipcf finished for chromosome arm 6q
multipcf finished for chromosome arm 7p
multipcf finished for chromosome arm 7q
multipcf finished for chromosome arm 8p
multipcf finished for chromosome arm 8q
multipcf finished for chromosome arm 9p
multipcf finished for chromosome arm 9q
multipcf finished for chromosome arm 10p
multipcf finished for chromosome arm 10q
multipcf finished for chromosome arm 11p
multipcf finished for chromosome arm 11q
multipcf finished for chromosome arm 12p
multipcf finished for chromosome arm 12q
multipcf finished for chromosome arm 13q
multipcf finished for chromosome arm 14q
multipcf finished for chromosome arm 15q
multipcf finished for chromosome arm 16p
multipcf finished for chromosome arm 16q
multipcf finished for chromosome arm 17p
multipcf finished for chromosome arm 17q
multipcf finished for chromosome arm 18p
multipcf finished for chromosome arm 18q
multipcf finished for chromosome arm 19p
multipcf finished for chromosome arm 19q
multipcf finished for chromosome arm 20p
multipcf finished for chromosome arm 20q
multipcf finished for chromosome arm 21q
multipcf finished for chromosome arm 22q
INFO [2021-10-15 04:21:40] ------------------------------------------------------------
INFO [2021-10-15 04:21:40] PureCN 1.22.2
INFO [2021-10-15 04:21:40] ------------------------------------------------------------
INFO [2021-10-15 04:21:40] Loading coverage files...
INFO [2021-10-15 04:21:40] seg.file and normal.coverage.file provided. Using both.
INFO [2021-10-15 04:21:40] Mean target coverages: 112X (tumor) 105X (normal).
WARN [2021-10-15 04:21:40] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:21:40] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:21:40] Removing 199 intervals with missing log.ratio.
INFO [2021-10-15 04:21:40] Using 9850 intervals (9850 on-target, 0 off-target).
INFO [2021-10-15 04:21:40] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:21:40] Loading VCF...
INFO [2021-10-15 04:21:41] Found 127 variants in VCF file.
INFO [2021-10-15 04:21:41] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:21:41] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:21:41] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:21:41] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:21:41] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:21:41] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:21:42] Total size of targeted genomic region: 1.68Mb (2.66Mb with 50bp padding).
INFO [2021-10-15 04:21:42] 1.0% of targets contain variants.
INFO [2021-10-15 04:21:42] Removing 0 variants outside intervals.
INFO [2021-10-15 04:21:42] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:21:42] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:21:42] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2021-10-15 04:21:42] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:21:42] Sample sex: ?
INFO [2021-10-15 04:21:42] Segmenting data...
INFO [2021-10-15 04:21:42] Loaded provided segmentation file file9cf86b0e2ac7.seg (format DNAcopy).
INFO [2021-10-15 04:21:42] Re-centering provided segment means (offset 0.0110).
INFO [2021-10-15 04:21:43] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:21:43] Found 59 segments with median size of 30.15Mb.
INFO [2021-10-15 04:21:43] Using 125 variants.
INFO [2021-10-15 04:21:43] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:21:43] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:21:44] Local optima: 0.6/1.9
INFO [2021-10-15 04:21:44] Testing local optimum 1/1 at purity 0.60 and total ploidy 1.90...
INFO [2021-10-15 04:21:45] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:21:45] Fitting variants for purity 0.60, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:21:47] Fitting variants for purity 0.40, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:21:50] Fitting variants for purity 0.45, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:21:52] Fitting variants for purity 0.50, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:21:55] Fitting variants for purity 0.55, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:21:57] Fitting variants for purity 0.65, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:22:00] Fitting variants for purity 0.70, tumor ploidy 1.76 and contamination 0.01.
INFO [2021-10-15 04:22:03] Optimized purity: 0.65
INFO [2021-10-15 04:22:03] Done.
INFO [2021-10-15 04:22:03] ------------------------------------------------------------
INFO [2021-10-15 04:22:05] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
FATAL [2021-10-15 04:22:05] Cannot find centromeres for hg20. Provide them manually or select a
FATAL [2021-10-15 04:22:05] supported genome.
FATAL [2021-10-15 04:22:05]
FATAL [2021-10-15 04:22:05] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:05] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:06] Found 20 variants in VCF file.
INFO [2021-10-15 04:22:06] 20 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:22:07] Found 127 variants in VCF file.
INFO [2021-10-15 04:22:07] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:22:08] ------------------------------------------------------------
INFO [2021-10-15 04:22:08] PureCN 1.22.2
INFO [2021-10-15 04:22:08] ------------------------------------------------------------
INFO [2021-10-15 04:22:08] Loading coverage files...
INFO [2021-10-15 04:22:08] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:22:08] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:22:08] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:22:08] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:22:08] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:22:08] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:22:08] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:22:08] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:22:08] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:22:08] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:22:08] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:22:08] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:22:08] Loading VCF...
INFO [2021-10-15 04:22:08] Found 127 variants in VCF file.
INFO [2021-10-15 04:22:09] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:22:09] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:22:09] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:22:09] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:22:09] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:22:09] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:22:09] Total size of targeted genomic region: 1.53Mb (2.33Mb with 50bp padding).
INFO [2021-10-15 04:22:10] 1.2% of targets contain variants.
INFO [2021-10-15 04:22:10] Removing 4 variants outside intervals.
INFO [2021-10-15 04:22:10] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:22:10] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:22:10] Found SOMATIC annotation in VCF. Setting mapping bias to 0.972.
INFO [2021-10-15 04:22:10] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:22:10] Sample sex: ?
INFO [2021-10-15 04:22:10] Segmenting data...
INFO [2021-10-15 04:22:11] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:22:11] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:22:12] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:22:12] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:22:12] Using 121 variants.
INFO [2021-10-15 04:22:12] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:22:12] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:22:12] Local optima: 0.65/1.8, 0.52/2
INFO [2021-10-15 04:22:12] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:22:13] Testing local optimum 2/2 at purity 0.52 and total ploidy 2.00...
INFO [2021-10-15 04:22:14] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:22:14] Fitting variants with beta model for local optimum 1/2...
INFO [2021-10-15 04:22:14] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:22:16] Optimized purity: 0.65
INFO [2021-10-15 04:22:16] Done.
INFO [2021-10-15 04:22:16] ------------------------------------------------------------
FATAL [2021-10-15 04:22:20] log.ratio NULL in .writeLogRatioFileGATK4
FATAL [2021-10-15 04:22:20]
FATAL [2021-10-15 04:22:20] This runtime error might be caused by invalid input data or parameters.
FATAL [2021-10-15 04:22:20] Please report bug (PureCN 1.22.2).
INFO [2021-10-15 04:22:20] Loaded provided segmentation file example_seg.txt (format DNAcopy).
INFO [2021-10-15 04:22:20] Re-centering provided segment means (offset -0.0033).
INFO [2021-10-15 04:22:20] Loaded provided segmentation file example_gatk4_modelfinal.seg.gz (format GATK4).
WARN [2021-10-15 04:22:20] Expecting numeric chromosome names in seg.file, assuming file is properly sorted.
INFO [2021-10-15 04:22:20] Re-centering provided segment means (offset -0.0037).
INFO [2021-10-15 04:22:21] 576 on-target bins with low coverage in all samples.
WARN [2021-10-15 04:22:21] You are likely not using the correct baits file!
WARN [2021-10-15 04:22:21] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:22:21] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:22:21] Processing on-target regions...
INFO [2021-10-15 04:22:22] Removing 930 intervals with low coverage in normalDB.
INFO [2021-10-15 04:22:22] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2021-10-15 04:22:24] ------------------------------------------------------------
INFO [2021-10-15 04:22:24] PureCN 1.22.2
INFO [2021-10-15 04:22:24] ------------------------------------------------------------
INFO [2021-10-15 04:22:24] Using BiocParallel for parallel optimization.
INFO [2021-10-15 04:22:24] Loading coverage files...
INFO [2021-10-15 04:22:24] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:22:24] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:22:24] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:22:24] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:22:24] Removing 705 intervals excluded in normalDB.
INFO [2021-10-15 04:22:24] normalDB provided. Setting minimum coverage for segmentation to 0.0015X.
INFO [2021-10-15 04:22:24] Removing 1066 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:22:24] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:22:24] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:22:25] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:22:25] Sample sex: ?
INFO [2021-10-15 04:22:25] Segmenting data...
INFO [2021-10-15 04:22:25] Interval weights found, will use weighted CBS.
INFO [2021-10-15 04:22:25] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:22:25] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2021-10-15 04:22:26] Found 54 segments with median size of 23.50Mb.
INFO [2021-10-15 04:22:26] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:22:26] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:22:26] Local optima: 0.65/1.8, 0.52/2
INFO [2021-10-15 04:22:55] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:22:55] Done.
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] Reading C:\Users\biocbuild\bbs-3.13-bioc\tmpdir\Rtmpemrbp3\file9cf818053865.rds...
FATAL [2021-10-15 04:22:55] runAbsoluteCN was run without a VCF file.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:22:55] runAbsoluteCN was run without a VCF file.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] Loading coverage files...
FATAL [2021-10-15 04:22:55] Need a normal coverage file if log.ratio and seg.file are not provided.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
FATAL [2021-10-15 04:22:55] min.ploidy or max.ploidy not within expected range.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
FATAL [2021-10-15 04:22:55] min.ploidy or max.ploidy not within expected range.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
FATAL [2021-10-15 04:22:55] test.num.copy not within expected range.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
WARN [2021-10-15 04:22:55] test.num.copy outside recommended range.
FATAL [2021-10-15 04:22:55] max.non.clonal not within expected range or format.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
INFO [2021-10-15 04:22:55] PureCN 1.22.2
INFO [2021-10-15 04:22:55] ------------------------------------------------------------
WARN [2021-10-15 04:22:55] test.num.copy outside recommended range.
FATAL [2021-10-15 04:22:55] max.non.clonal not within expected range or format.
FATAL [2021-10-15 04:22:55]
FATAL [2021-10-15 04:22:55] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:55] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
FATAL [2021-10-15 04:22:56] test.purity not within expected range.
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] Loading coverage files...
FATAL [2021-10-15 04:22:56] Tumor and normal are identical. This won't give any meaningful results
FATAL [2021-10-15 04:22:56] and I'm stopping here.
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] Loading coverage files...
FATAL [2021-10-15 04:22:56] Length of log.ratio different from tumor coverage.
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
FATAL [2021-10-15 04:22:56] prior.purity must have the same length as test.purity.
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
FATAL [2021-10-15 04:22:56] min.gof not within expected range or format.
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
FATAL [2021-10-15 04:22:56] prior.purity not within expected range or format.
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
INFO [2021-10-15 04:22:56] PureCN 1.22.2
INFO [2021-10-15 04:22:56] ------------------------------------------------------------
FATAL [2021-10-15 04:22:56] prior.purity must add to 1. Sum is 1.5
FATAL [2021-10-15 04:22:56]
FATAL [2021-10-15 04:22:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
FATAL [2021-10-15 04:22:57] max.homozygous.loss not within expected range or format.
FATAL [2021-10-15 04:22:57]
FATAL [2021-10-15 04:22:57] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:57] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
FATAL [2021-10-15 04:22:57] prior.K not within expected range or format.
FATAL [2021-10-15 04:22:57]
FATAL [2021-10-15 04:22:57] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:57] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
FATAL [2021-10-15 04:22:57] prior.contamination not within expected range or format.
FATAL [2021-10-15 04:22:57]
FATAL [2021-10-15 04:22:57] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:57] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
FATAL [2021-10-15 04:22:57] Iterations not in the expected range from 10 to 250.
FATAL [2021-10-15 04:22:57]
FATAL [2021-10-15 04:22:57] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:57] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
FATAL [2021-10-15 04:22:57] Iterations not in the expected range from 10 to 250.
FATAL [2021-10-15 04:22:57]
FATAL [2021-10-15 04:22:57] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:57] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] Loading coverage files...
FATAL [2021-10-15 04:22:57] Missing tumor.coverage.file requires seg.file or log.ratio and
FATAL [2021-10-15 04:22:57] interval.file.
FATAL [2021-10-15 04:22:57]
FATAL [2021-10-15 04:22:57] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:57] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] PureCN 1.22.2
INFO [2021-10-15 04:22:57] ------------------------------------------------------------
INFO [2021-10-15 04:22:57] Loading coverage files...
FATAL [2021-10-15 04:22:58] Interval files in normal and tumor different.
FATAL [2021-10-15 04:22:58]
FATAL [2021-10-15 04:22:58] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:58] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:58] ------------------------------------------------------------
INFO [2021-10-15 04:22:58] PureCN 1.22.2
INFO [2021-10-15 04:22:58] ------------------------------------------------------------
INFO [2021-10-15 04:22:58] Loading coverage files...
INFO [2021-10-15 04:22:58] Mean target coverages: 0X (tumor) 99X (normal).
WARN [2021-10-15 04:22:58] Large difference in coverage of tumor and normal.
FATAL [2021-10-15 04:22:58] No finite intervals.
FATAL [2021-10-15 04:22:58]
FATAL [2021-10-15 04:22:58] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:22:58] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:22:58] ------------------------------------------------------------
INFO [2021-10-15 04:22:58] PureCN 1.22.2
INFO [2021-10-15 04:22:58] ------------------------------------------------------------
INFO [2021-10-15 04:22:58] Loading coverage files...
INFO [2021-10-15 04:22:59] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:22:59] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:22:59] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:22:59] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:22:59] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:22:59] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:22:59] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:22:59] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:22:59] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:22:59] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:22:59] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:22:59] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:22:59] Loading VCF...
INFO [2021-10-15 04:23:00] Found 127 variants in VCF file.
INFO [2021-10-15 04:23:00] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:23:00] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:23:00] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:23:00] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:23:01] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'con' in selecting a method for function 'import': Format 'txt' unsupported
FATAL [2021-10-15 04:23:01] Could not import snp.blacklist
FATAL [2021-10-15 04:23:01] C:/Users/biocbuild/bbs-3.13-bioc/R/library/PureCN/extdata/example_normal.txt:Error
FATAL [2021-10-15 04:23:01] in h(simpleError(msg, call)) : error in evaluating the argument 'con'
FATAL [2021-10-15 04:23:01] in selecting a method for function 'import': Format 'txt' unsupported
FATAL [2021-10-15 04:23:01]
FATAL [2021-10-15 04:23:01] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:23:01] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:23:01] ------------------------------------------------------------
INFO [2021-10-15 04:23:01] PureCN 1.22.2
INFO [2021-10-15 04:23:01] ------------------------------------------------------------
INFO [2021-10-15 04:23:01] Loading coverage files...
INFO [2021-10-15 04:23:01] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:23:01] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:23:01] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:23:01] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:23:01] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:23:01] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:23:01] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:23:01] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:23:01] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2021-10-15 04:23:01] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:23:01] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:23:01] Loading VCF...
INFO [2021-10-15 04:23:02] Found 127 variants in VCF file.
INFO [2021-10-15 04:23:02] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:23:02] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:23:02] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:23:02] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:23:03] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:23:03] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:23:03] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2021-10-15 04:23:03] 1.0% of targets contain variants.
INFO [2021-10-15 04:23:03] Removing 2 variants outside intervals.
INFO [2021-10-15 04:23:04] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:23:04] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:23:04] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2021-10-15 04:23:04] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:23:04] Sample sex: ?
INFO [2021-10-15 04:23:04] Segmenting data...
INFO [2021-10-15 04:23:04] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:23:04] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:23:05] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:23:06] Found 54 segments with median size of 24.88Mb.
INFO [2021-10-15 04:23:06] Using 123 variants.
INFO [2021-10-15 04:23:06] Mean standard deviation of log-ratios: 0.37
INFO [2021-10-15 04:23:06] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:23:07] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2021-10-15 04:23:07] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2021-10-15 04:23:08] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:09] Recalibrating log-ratios...
INFO [2021-10-15 04:23:09] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:09] Recalibrating log-ratios...
INFO [2021-10-15 04:23:09] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:09] Recalibrating log-ratios...
INFO [2021-10-15 04:23:09] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:10] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:10] Recalibrating log-ratios...
INFO [2021-10-15 04:23:10] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:10] Recalibrating log-ratios...
INFO [2021-10-15 04:23:10] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:11] Recalibrating log-ratios...
INFO [2021-10-15 04:23:11] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:11] Skipping 1 solutions that converged to the same optima.
INFO [2021-10-15 04:23:11] Skipping 1 solutions exceeding max.non.clonal (0.20): 0.3/1.9 (purity/tumor ploidy)
INFO [2021-10-15 04:23:11] Fitting variants with beta model for local optimum 1/3...
INFO [2021-10-15 04:23:11] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2021-10-15 04:23:14] Optimized purity: 0.65
INFO [2021-10-15 04:23:14] Done.
INFO [2021-10-15 04:23:14] ------------------------------------------------------------
INFO [2021-10-15 04:23:14] ------------------------------------------------------------
INFO [2021-10-15 04:23:14] PureCN 1.22.2
INFO [2021-10-15 04:23:14] ------------------------------------------------------------
INFO [2021-10-15 04:23:14] Loading coverage files...
INFO [2021-10-15 04:23:15] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:23:15] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:23:15] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:23:15] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:23:15] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:23:15] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:23:15] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:23:15] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:23:15] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:23:15] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:23:15] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:23:15] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:23:15] Loading VCF...
INFO [2021-10-15 04:23:15] Found 127 variants in VCF file.
WARN [2021-10-15 04:23:15] DP FORMAT field contains NAs. Removing 3 variants.
WARN [2021-10-15 04:23:15] DB INFO flag contains NAs
INFO [2021-10-15 04:23:15] 119 (96.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:23:16] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:23:16] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:23:16] Removing 1 variants with AF < 0.030 or AF >= 0.970 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:23:16] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:23:16] Total size of targeted genomic region: 1.53Mb (2.33Mb with 50bp padding).
INFO [2021-10-15 04:23:17] 1.2% of targets contain variants.
INFO [2021-10-15 04:23:17] Setting somatic prior probabilities for dbSNP hits to 0.000500 or to 0.500000 otherwise.
INFO [2021-10-15 04:23:17] Excluding 5 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:23:17] Sample sex: ?
INFO [2021-10-15 04:23:17] Segmenting data...
INFO [2021-10-15 04:23:17] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:23:17] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2021-10-15 04:23:19] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:23:19] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:23:19] Using 123 variants.
INFO [2021-10-15 04:23:19] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:23:19] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:23:20] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2021-10-15 04:23:20] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2021-10-15 04:23:21] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:21] Recalibrating log-ratios...
INFO [2021-10-15 04:23:21] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:22] Recalibrating log-ratios...
INFO [2021-10-15 04:23:22] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:22] Recalibrating log-ratios...
INFO [2021-10-15 04:23:22] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2021-10-15 04:23:22] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:23] Recalibrating log-ratios...
INFO [2021-10-15 04:23:23] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:23] Recalibrating log-ratios...
INFO [2021-10-15 04:23:23] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:23] Recalibrating log-ratios...
INFO [2021-10-15 04:23:23] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2021-10-15 04:23:24] Skipping 2 solutions exceeding max.non.clonal (0.20): 0.3/1.9, 0.35/1.67 (purity/tumor ploidy)
INFO [2021-10-15 04:23:24] Fitting variants with beta model for local optimum 1/3...
INFO [2021-10-15 04:23:24] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:23:27] Optimized purity: 0.65
INFO [2021-10-15 04:23:27] Done.
INFO [2021-10-15 04:23:27] ------------------------------------------------------------
INFO [2021-10-15 04:23:27] ------------------------------------------------------------
INFO [2021-10-15 04:23:27] PureCN 1.22.2
INFO [2021-10-15 04:23:27] ------------------------------------------------------------
INFO [2021-10-15 04:23:27] Loading coverage files...
INFO [2021-10-15 04:23:27] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:23:28] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:23:28] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:23:28] No Gene column in interval.file. You won't get gene-level calls.
INFO [2021-10-15 04:23:28] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:23:28] Removing 15 low/high GC targets.
INFO [2021-10-15 04:23:28] Removing 21 small (< 5bp) intervals.
INFO [2021-10-15 04:23:28] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:23:28] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:23:28] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:23:28] Removing 1492 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:23:28] Removing 36 low mappability intervals.
INFO [2021-10-15 04:23:28] Using 8006 intervals (8006 on-target, 0 off-target).
INFO [2021-10-15 04:23:29] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:23:29] AT/GC dropout: 1.01 (tumor), 1.03 (normal), 0.98 (coverage log-ratio).
INFO [2021-10-15 04:23:29] Loading VCF...
INFO [2021-10-15 04:23:29] Found 127 variants in VCF file.
INFO [2021-10-15 04:23:29] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:23:29] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:23:29] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:23:30] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:23:30] Removing 1 variants with AF < 0.030 or AF >= Inf or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:23:30] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:23:30] Total size of targeted genomic region: 1.51Mb (2.31Mb with 50bp padding).
INFO [2021-10-15 04:23:30] 1.2% of targets contain variants.
INFO [2021-10-15 04:23:31] Removing 11 variants outside intervals.
INFO [2021-10-15 04:23:31] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:23:31] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:23:31] Found SOMATIC annotation in VCF. Setting mapping bias to 0.970.
INFO [2021-10-15 04:23:31] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:23:31] Sample sex: ?
INFO [2021-10-15 04:23:31] Segmenting data...
INFO [2021-10-15 04:23:32] Using unweighted PSCBS.
INFO [2021-10-15 04:23:32] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:23:47] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:23:47] Found 72 segments with median size of 27.25Mb.
INFO [2021-10-15 04:23:47] Using 114 variants.
INFO [2021-10-15 04:23:47] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:23:47] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:23:47] Local optima: 0.65/1.8, 0.38/2.2
INFO [2021-10-15 04:23:47] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:23:47] Recalibrating log-ratios...
INFO [2021-10-15 04:23:47] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:23:48] Recalibrating log-ratios...
INFO [2021-10-15 04:23:48] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:23:48] Recalibrating log-ratios...
INFO [2021-10-15 04:23:48] Testing local optimum 1/2 at purity 0.65 and total ploidy 1.80...
INFO [2021-10-15 04:23:49] Testing local optimum 2/2 at purity 0.38 and total ploidy 2.20...
INFO [2021-10-15 04:23:50] Skipping 1 solutions exceeding max.non.clonal (0.20): 0.65/2.06 (purity/tumor ploidy)
INFO [2021-10-15 04:23:50] Fitting variants with beta model for local optimum 2/2...
INFO [2021-10-15 04:23:50] Fitting variants for purity 0.40, tumor ploidy 2.55 and contamination 0.01.
INFO [2021-10-15 04:23:53] Optimized purity: 0.40
INFO [2021-10-15 04:23:53] Done.
INFO [2021-10-15 04:23:53] ------------------------------------------------------------
FATAL [2021-10-15 04:23:53] This function requires gene-level calls. Please add a column 'Gene'
FATAL [2021-10-15 04:23:53] containing gene symbols to the interval.file.
FATAL [2021-10-15 04:23:53]
FATAL [2021-10-15 04:23:53] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:23:53] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:23:55] ------------------------------------------------------------
INFO [2021-10-15 04:23:55] PureCN 1.22.2
INFO [2021-10-15 04:23:55] ------------------------------------------------------------
INFO [2021-10-15 04:23:55] Loading coverage files...
INFO [2021-10-15 04:23:55] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:23:55] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:23:55] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:23:55] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:23:55] Removing 22 small (< 5bp) intervals.
INFO [2021-10-15 04:23:55] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:23:55] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:23:55] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:23:55] Removing 1497 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:23:55] Using 8050 intervals (8050 on-target, 0 off-target).
INFO [2021-10-15 04:23:55] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:23:55] No interval.file provided. Cannot check for any GC-biases.
INFO [2021-10-15 04:23:55] Loading VCF...
INFO [2021-10-15 04:23:55] Found 127 variants in VCF file.
INFO [2021-10-15 04:23:56] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
FATAL [2021-10-15 04:23:56] Different chromosome names in coverage and VCF.
FATAL [2021-10-15 04:23:56]
FATAL [2021-10-15 04:23:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:23:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:23:56] ------------------------------------------------------------
INFO [2021-10-15 04:23:56] PureCN 1.22.2
INFO [2021-10-15 04:23:56] ------------------------------------------------------------
INFO [2021-10-15 04:23:56] Loading coverage files...
INFO [2021-10-15 04:23:56] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:23:56] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:23:56] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:23:56] tumor.coverage.file and interval.file do not align.
FATAL [2021-10-15 04:23:56]
FATAL [2021-10-15 04:23:56] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:23:56] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:23:57] ------------------------------------------------------------
INFO [2021-10-15 04:23:57] PureCN 1.22.2
INFO [2021-10-15 04:23:57] ------------------------------------------------------------
INFO [2021-10-15 04:23:57] Loading coverage files...
INFO [2021-10-15 04:23:57] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:23:57] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:23:57] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:23:58] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:23:58] Removing 15 low/high GC targets.
INFO [2021-10-15 04:23:58] Removing 21 small (< 5bp) intervals.
INFO [2021-10-15 04:23:58] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:23:58] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:23:58] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:23:58] Removing 1492 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:23:58] Removing 36 low mappability intervals.
INFO [2021-10-15 04:23:58] Using 8006 intervals (8006 on-target, 0 off-target).
INFO [2021-10-15 04:23:58] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:23:58] AT/GC dropout: 1.01 (tumor), 1.03 (normal), 0.98 (coverage log-ratio).
INFO [2021-10-15 04:23:58] Loading VCF...
INFO [2021-10-15 04:23:59] Found 127 variants in VCF file.
INFO [2021-10-15 04:23:59] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:23:59] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:23:59] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:23:59] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:23:59] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:24:00] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:24:00] Total size of targeted genomic region: 1.51Mb (2.31Mb with 50bp padding).
INFO [2021-10-15 04:24:00] 1.2% of targets contain variants.
INFO [2021-10-15 04:24:00] Removing 11 variants outside intervals.
INFO [2021-10-15 04:24:00] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:24:00] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:24:00] Found SOMATIC annotation in VCF. Setting mapping bias to 0.970.
INFO [2021-10-15 04:24:00] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:24:01] Sample sex: ?
INFO [2021-10-15 04:24:01] Segmenting data...
INFO [2021-10-15 04:24:01] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:24:01] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:24:02] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:24:02] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:24:02] Using 114 variants.
INFO [2021-10-15 04:24:02] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:24:02] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:24:03] Local optima: 0.63/1.9
INFO [2021-10-15 04:24:03] Testing local optimum 1/1 at purity 0.63 and total ploidy 1.90...
INFO [2021-10-15 04:24:04] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:24:04] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:24:06] Optimized purity: 0.65
INFO [2021-10-15 04:24:06] Done.
INFO [2021-10-15 04:24:06] ------------------------------------------------------------
INFO [2021-10-15 04:24:06] ------------------------------------------------------------
INFO [2021-10-15 04:24:06] PureCN 1.22.2
INFO [2021-10-15 04:24:06] ------------------------------------------------------------
INFO [2021-10-15 04:24:06] Loading coverage files...
INFO [2021-10-15 04:24:06] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2021-10-15 04:24:07] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:07] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:24:08] Removing 228 intervals with missing log.ratio.
INFO [2021-10-15 04:24:08] Removing 15 low/high GC targets.
INFO [2021-10-15 04:24:08] Removing 21 small (< 5bp) intervals.
INFO [2021-10-15 04:24:08] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2021-10-15 04:24:08] No normalDB provided. Provide one for better results.
INFO [2021-10-15 04:24:08] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2021-10-15 04:24:08] Removing 1492 low count (< 100 total reads) intervals.
INFO [2021-10-15 04:24:08] Removing 36 low mappability intervals.
INFO [2021-10-15 04:24:08] Using 8006 intervals (8006 on-target, 0 off-target).
INFO [2021-10-15 04:24:08] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:24:08] AT/GC dropout: 1.01 (tumor), 1.03 (normal), 0.98 (coverage log-ratio).
INFO [2021-10-15 04:24:08] Loading VCF...
INFO [2021-10-15 04:24:08] Found 127 variants in VCF file.
INFO [2021-10-15 04:24:08] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:24:09] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:24:09] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:24:09] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:24:09] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:24:09] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:24:09] Total size of targeted genomic region: 1.51Mb (2.31Mb with 50bp padding).
INFO [2021-10-15 04:24:10] 1.2% of targets contain variants.
INFO [2021-10-15 04:24:10] Removing 11 variants outside intervals.
INFO [2021-10-15 04:24:10] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:24:10] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:24:10] Found SOMATIC annotation in VCF. Setting mapping bias to 0.970.
INFO [2021-10-15 04:24:10] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:24:10] Sample sex: ?
INFO [2021-10-15 04:24:10] Segmenting data...
INFO [2021-10-15 04:24:10] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:24:10] Setting undo.SD parameter to 1.000000.
INFO [2021-10-15 04:24:11] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:24:12] Found 59 segments with median size of 17.67Mb.
INFO [2021-10-15 04:24:12] Using 114 variants.
INFO [2021-10-15 04:24:12] Mean standard deviation of log-ratios: 0.34
INFO [2021-10-15 04:24:12] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:24:12] Local optima: 0.63/1.9
INFO [2021-10-15 04:24:12] Testing local optimum 1/1 at purity 0.63 and total ploidy 1.90...
INFO [2021-10-15 04:24:13] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:24:14] Fitting variants for purity 0.65, tumor ploidy 1.75 and contamination 0.01.
INFO [2021-10-15 04:24:16] Optimized purity: 0.65
INFO [2021-10-15 04:24:16] Done.
INFO [2021-10-15 04:24:16] ------------------------------------------------------------
FATAL [2021-10-15 04:24:16] chr1 not valid chromosome name(s). Valid names are:
FATAL [2021-10-15 04:24:16] 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22
FATAL [2021-10-15 04:24:16]
FATAL [2021-10-15 04:24:16] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:24:16] parameters (PureCN 1.22.2).
INFO [2021-10-15 04:24:16] ------------------------------------------------------------
INFO [2021-10-15 04:24:16] PureCN 1.22.2
INFO [2021-10-15 04:24:16] ------------------------------------------------------------
INFO [2021-10-15 04:24:16] Loading coverage files...
WARN [2021-10-15 04:24:17] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
WARN [2021-10-15 04:24:17] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:17] Allosome coverage missing, cannot determine sex.
INFO [2021-10-15 04:24:17] Removing 10 intervals with missing log.ratio.
INFO [2021-10-15 04:24:17] Using 10039 intervals (10039 on-target, 0 off-target).
INFO [2021-10-15 04:24:17] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2021-10-15 04:24:17] Loading VCF...
INFO [2021-10-15 04:24:17] Found 127 variants in VCF file.
INFO [2021-10-15 04:24:17] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2021-10-15 04:24:18] LIB-02240e4 is tumor in VCF file.
INFO [2021-10-15 04:24:18] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2021-10-15 04:24:18] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2021-10-15 04:24:18] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2021-10-15 04:24:18] Removing 0 low quality variants with BQ < 25.
INFO [2021-10-15 04:24:18] Total size of targeted genomic region: 1.71Mb (2.70Mb with 50bp padding).
INFO [2021-10-15 04:24:19] 1.0% of targets contain variants.
INFO [2021-10-15 04:24:19] Removing 0 variants outside intervals.
INFO [2021-10-15 04:24:19] Found SOMATIC annotation in VCF.
INFO [2021-10-15 04:24:19] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2021-10-15 04:24:19] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2021-10-15 04:24:19] Excluding 0 novel or poor quality variants from segmentation.
INFO [2021-10-15 04:24:19] Sample sex: ?
INFO [2021-10-15 04:24:19] Segmenting data...
INFO [2021-10-15 04:24:19] Loaded provided segmentation file example_seg.txt (format DNAcopy).
WARN [2021-10-15 04:24:19] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
INFO [2021-10-15 04:24:19] Re-centering provided segment means (offset -0.0033).
INFO [2021-10-15 04:24:19] Loading pre-computed boundaries for DNAcopy...
INFO [2021-10-15 04:24:19] Setting undo.SD parameter to 0.000000.
Setting multi-figure configuration
INFO [2021-10-15 04:24:20] Setting prune.hclust.h parameter to 0.200000.
INFO [2021-10-15 04:24:20] Found 54 segments with median size of 24.88Mb.
INFO [2021-10-15 04:24:20] Using 125 variants.
INFO [2021-10-15 04:24:20] Mean standard deviation of log-ratios: 0.40
INFO [2021-10-15 04:24:20] 2D-grid search of purity and ploidy...
INFO [2021-10-15 04:24:21] Local optima: 0.6/1.9
INFO [2021-10-15 04:24:21] Testing local optimum 1/1 at purity 0.60 and total ploidy 1.90...
INFO [2021-10-15 04:24:22] Fitting variants with beta model for local optimum 1/1...
INFO [2021-10-15 04:24:22] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2021-10-15 04:24:25] Optimized purity: 0.65
INFO [2021-10-15 04:24:25] Done.
INFO [2021-10-15 04:24:25] ------------------------------------------------------------
WARN [2021-10-15 04:24:26] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:26] Allosome coverage missing, cannot determine sex.
Setting multi-figure configuration
FATAL [2021-10-15 04:24:35] Segmentation file expected with colnames ID, chrom, loc.start, loc.end,
FATAL [2021-10-15 04:24:35] num.mark, seg.mean
FATAL [2021-10-15 04:24:35]
FATAL [2021-10-15 04:24:35] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:24:35] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:24:36] seg.file contains multiple samples and sampleid missing.
FATAL [2021-10-15 04:24:36]
FATAL [2021-10-15 04:24:36] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:24:36] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:24:36] seg.file contains multiple samples and sampleid does not match any.
FATAL [2021-10-15 04:24:36]
FATAL [2021-10-15 04:24:36] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:24:36] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:24:36] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:36] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:46] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:46] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:46] Provided sampleid (example_tumor.txt) does not match Sample1 found in segmentation.
WARN [2021-10-15 04:24:50] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:50] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:24:51] No normalDB provided. Provide one for better results.
WARN [2021-10-15 04:25:00] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:00] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:04] Provided sampleid (Sample.1) does not match Sample1 found in segmentation.
Setting multi-figure configuration
WARN [2021-10-15 04:25:13] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:13] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:13] No normalDB provided. Provide one for better results.
WARN [2021-10-15 04:25:13] Sampleid looks like a normal in VCF, not like a tumor.
WARN [2021-10-15 04:25:21] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:21] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:25:21] normalDB not a valid normalDB object. Use createNormalDatabase to
FATAL [2021-10-15 04:25:21] create one.
FATAL [2021-10-15 04:25:21]
FATAL [2021-10-15 04:25:21] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:25:21] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:25:22] You are likely not using the correct baits file!
WARN [2021-10-15 04:25:22] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:22] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:26] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:26] Allosome coverage missing, cannot determine sex.
FATAL [2021-10-15 04:25:26] normalDB appears to be empty.
FATAL [2021-10-15 04:25:26]
FATAL [2021-10-15 04:25:26] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:25:26] parameters (PureCN 1.22.2).
WARN [2021-10-15 04:25:26] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:26] Allosome coverage missing, cannot determine sex.
WARN [2021-10-15 04:25:27] Intervals in coverage and interval.file have conflicting on/off-target annotation.
WARN [2021-10-15 04:25:43] Cannot find gatk binary in path.
FATAL [2021-10-15 04:26:30] The normal.panel.vcf.file contains only a single sample.
FATAL [2021-10-15 04:26:30]
FATAL [2021-10-15 04:26:30] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:26:30] parameters (PureCN 1.22.2).
FATAL [2021-10-15 04:26:31] mapping.bias.file must be a file with *.rds suffix.
FATAL [2021-10-15 04:26:31]
FATAL [2021-10-15 04:26:31] This is most likely a user error due to invalid input data or
FATAL [2021-10-15 04:26:31] parameters (PureCN 1.22.2).
Failed with error: 'there is no package called 'genomicsdb''
== Skipped tests ===============================================================
* gatk binary > 4.1.7.0 required (1)
* genomicsdb required (1)
[ FAIL 0 | WARN 54 | SKIP 2 | PASS 367 ]
>
> proc.time()
user system elapsed
464.34 2.84 569.15
|
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PureCN.Rcheck/examples_i386/PureCN-Ex.timings
|
PureCN.Rcheck/examples_x64/PureCN-Ex.timings
|