## ----installation, echo=TRUE, eval=FALSE--------------------------------- # library(devtools) # install_github("wjawaid/roots") ## ----example, echo=TRUE, eval=FALSE-------------------------------------- # library(roots) # # ## Load data # blood <- read.table("http://blood.stemcells.cam.ac.uk/data/norm_counts_nestorowa_data.txt", sep = " ") # cellNames <- read.table("http://blood.stemcells.cam.ac.uk/data/cell_names_nestorowa_data.txt", sep = " ", stringsAsFactors = FALSE)[,1] # rownames(blood) <- gsub("LT\\.", "LT-", cellNames) # geneNames <- read.table("http://blood.stemcells.cam.ac.uk/data/gene_names_nestorowa_data.txt", sep = " ", stringsAsFactors = FALSE)[,1] # colnames(blood) <- geneNames # blood <- as.matrix(blood) # rm(cellNames, geneNames) # # ## Load metadata # meta <- read.csv("http://blood.stemcells.cam.ac.uk/data/wj_out_jd.csv") # colnames(meta) <- c("cellType", "index", "name") # rownames(meta) <- meta$name # meta$col <- bglab::ggCol(meta$cellType) # nmeta <- data.frame(col=rep("#00000011", nrow(blood)), stringsAsFactors = FALSE, row.names = rownames(blood)) # nmeta[rownames(meta),"col"] <- meta$col # leg <- data.frame(cell=as.character(unique(meta$cellType)), col=as.character(unique(meta$col)), stringsAsFactors = FALSE) # legOrd <- c(5, 8, 6, 7, 1, 4, 2, 3) # # ## Analyse # xx <- goggles(blood) # # ## Plot # plot(xx$l, pch=16, col = nmeta[rownames(xx$l), "col"]) # legend("topright", legend = leg$cell[legOrd], fill=leg$col[legOrd], inset=0.02) #