1 Basics

1.1 Install chevreulProcess

R is an open-source statistical environment which can be easily modified to enhance its functionality via packages. chevreulProcess is a R package available via the Bioconductor repository for packages. R can be installed on any operating system from CRAN after which you can install chevreulProcess by using the following commands in your R session:

if (!requireNamespace("BiocManager", quietly = TRUE)) {
    install.packages("BiocManager")
}

BiocManager::install("chevreulProcess")

1.2 Required knowledge

The chevreulProcess package is designed for single-cell RNA sequencing data. The functions included within this package are derived from other packages that have implemented the infrastructure needed for RNA-seq data processing and analysis. Packages that have been instrumental in the development of chevreulProcess include, Biocpkg("SummarizedExperiment") and Biocpkg("scater").

1.3 Asking for help

R and Bioconductor have a steep learning curve so it is critical to learn where to ask for help. The Bioconductor support site is the main resource for getting help: remember to use the chevreulProcess tag and check the older posts.

2 Quick start to using chevreulProcess

The chevreulProcess package contains functions to preprocess, cluster, visualize, and perform other analyses on scRNA-seq data. It also contains a shiny app for easy visualization and analysis of scRNA data.

chvereul uses SingelCellExperiment (SCE) object type (from SingleCellExperiment) to store expression and other metadata from single-cell experiments.

This package features functions capable of:

  • Performing Clustering at a range of resolutions and Dimensional reduction of Raw Sequencing Data.
  • Visualizing scRNA data using different plotting functions.
  • Integration of multiple datasets for consistent analyses.
  • Cell cycle state regression and labeling.

library("chevreulProcess")

# Load the data
data("small_example_dataset")

R session information.

#> R version 4.6.0 RC (2026-04-17 r89917)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 24.04.4 LTS
#> 
#> Matrix products: default
#> BLAS:   /home/biocbuild/bbs-3.24-bioc/R/lib/libRblas.so 
#> LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.12.0  LAPACK version 3.12.0
#> 
#> locale:
#>  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
#>  [3] LC_TIME=en_GB              LC_COLLATE=C              
#>  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
#>  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
#>  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
#> [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
#> 
#> time zone: America/New_York
#> tzcode source: system (glibc)
#> 
#> attached base packages:
#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
#> [8] base     
#> 
#> other attached packages:
#>  [1] chevreulProcess_1.3.0       scater_1.39.4              
#>  [3] ggplot2_4.0.2               scuttle_1.21.6             
#>  [5] SingleCellExperiment_1.33.2 SummarizedExperiment_1.41.1
#>  [7] Biobase_2.71.0              GenomicRanges_1.63.2       
#>  [9] Seqinfo_1.1.0               IRanges_2.45.0             
#> [11] S4Vectors_0.49.2            BiocGenerics_0.57.1        
#> [13] generics_0.1.4              MatrixGenerics_1.23.0      
#> [15] matrixStats_1.5.0           BiocStyle_2.39.0           
#> 
#> loaded via a namespace (and not attached):
#>   [1] RColorBrewer_1.1-3        jsonlite_2.0.0           
#>   [3] shape_1.4.6.1             magrittr_2.0.5           
#>   [5] ggbeeswarm_0.7.3          GenomicFeatures_1.63.2   
#>   [7] farver_2.1.2              rmarkdown_2.31           
#>   [9] GlobalOptions_0.1.4       fs_2.1.0                 
#>  [11] BiocIO_1.21.0             vctrs_0.7.3              
#>  [13] memoise_2.0.1             Rsamtools_2.27.2         
#>  [15] DelayedMatrixStats_1.33.0 RCurl_1.98-1.18          
#>  [17] htmltools_0.5.9           S4Arrays_1.11.1          
#>  [19] curl_7.0.0                BiocNeighbors_2.5.4      
#>  [21] SparseArray_1.11.13       sass_0.4.10              
#>  [23] bslib_0.10.0              cachem_1.1.0             
#>  [25] ResidualMatrix_1.21.0     GenomicAlignments_1.47.0 
#>  [27] igraph_2.2.3              lifecycle_1.0.5          
#>  [29] pkgconfig_2.0.3           rsvd_1.0.5               
#>  [31] Matrix_1.7-5              R6_2.6.1                 
#>  [33] fastmap_1.2.0             digest_0.6.39            
#>  [35] colorspace_2.1-2          AnnotationDbi_1.73.1     
#>  [37] dqrng_0.4.1               irlba_2.3.7              
#>  [39] RSQLite_2.4.6             beachmat_2.27.5          
#>  [41] httr_1.4.8                abind_1.4-8              
#>  [43] compiler_4.6.0            bit64_4.6.0-1            
#>  [45] withr_3.0.2               S7_0.2.1-1               
#>  [47] BiocParallel_1.45.0       viridis_0.6.5            
#>  [49] DBI_1.3.0                 DelayedArray_0.37.1      
#>  [51] rjson_0.2.23              bluster_1.21.1           
#>  [53] tools_4.6.0               vipor_0.4.7              
#>  [55] otel_0.2.0                beeswarm_0.4.0           
#>  [57] glue_1.8.1                restfulr_0.0.16          
#>  [59] batchelor_1.27.1          grid_4.6.0               
#>  [61] cluster_2.1.8.2           megadepth_1.21.0         
#>  [63] gtable_0.3.6              tzdb_0.5.0               
#>  [65] ensembldb_2.35.0          hms_1.1.4                
#>  [67] metapod_1.19.2            BiocSingular_1.27.1      
#>  [69] ScaledMatrix_1.19.0       XVector_0.51.0           
#>  [71] stringr_1.6.0             ggrepel_0.9.8            
#>  [73] pillar_1.11.1             limma_3.67.1             
#>  [75] circlize_0.4.18           dplyr_1.2.1              
#>  [77] lattice_0.22-9            rtracklayer_1.71.3       
#>  [79] bit_4.6.0                 tidyselect_1.2.1         
#>  [81] locfit_1.5-9.12           Biostrings_2.79.5        
#>  [83] knitr_1.51                gridExtra_2.3            
#>  [85] bookdown_0.46             ProtGenerics_1.43.0      
#>  [87] edgeR_4.9.8               cmdfun_1.0.2             
#>  [89] xfun_0.57                 statmod_1.5.1            
#>  [91] stringi_1.8.7             UCSC.utils_1.7.1         
#>  [93] EnsDb.Hsapiens.v86_2.99.0 lazyeval_0.2.3           
#>  [95] yaml_2.3.12               evaluate_1.0.5           
#>  [97] codetools_0.2-20          cigarillo_1.1.0          
#>  [99] tibble_3.3.1              BiocManager_1.30.27      
#> [101] cli_3.6.6                 jquerylib_0.1.4          
#> [103] dichromat_2.0-0.1         Rcpp_1.1.1-1             
#> [105] GenomeInfoDb_1.47.2       png_0.1-9                
#> [107] XML_3.99-0.23             parallel_4.6.0           
#> [109] readr_2.2.0               blob_1.3.0               
#> [111] AnnotationFilter_1.35.0   scran_1.39.2             
#> [113] sparseMatrixStats_1.23.0  bitops_1.0-9             
#> [115] viridisLite_0.4.3         scales_1.4.0             
#> [117] purrr_1.2.2               crayon_1.5.3             
#> [119] rlang_1.2.0               KEGGREST_1.51.1