1 Basics

1.1 Install chevreulPlot

R is an open-source statistical environment which can be easily modified to enhance its functionality via packages. chevreulPlot is a R package available via the Bioconductor repository for packages. R can be installed on any operating system from CRAN after which you can install chevreulPlot by using the following commands in your R session:

if (!requireNamespace("BiocManager", quietly = TRUE)) {
    install.packages("BiocManager")
}

BiocManager::install("chevreulPlot")

1.2 Required knowledge

The chevreulPlot package is designed for single-cell RNA sequencing data. The functions included within this package are derived from other packages that have implemented the infrastructure needed for RNA-seq data processing and analysis. Packages that have been instrumental in the development of chevreulPlot include, Biocpkg("SummarizedExperiment") and Biocpkg("scater").

1.3 Asking for help

R and Bioconductor have a steep learning curve so it is critical to learn where to ask for help. The Bioconductor support site is the main resource for getting help: remember to use the chevreulPlot tag and check the older posts.

2 Quick start to using chevreulPlot

The chevreulPlot package contains functions to preprocess, cluster, visualize, and perform other analyses on scRNA-seq data. It also contains a shiny app for easy visualization and analysis of scRNA data.

chvereul uses SingelCellExperiment (SCE) object type (from SingleCellExperiment) to store expression and other metadata from single-cell experiments.

This package features functions capable of:

  • Performing Clustering at a range of resolutions and Dimensional reduction of Raw Sequencing Data.
  • Visualizing scRNA data using different plotting functions.
  • Integration of multiple datasets for consistent analyses.
  • Cell cycle state regression and labeling.

library("chevreulPlot")

# Load the data
data("small_example_dataset")
sessionInfo()
#> R version 4.6.0 RC (2026-04-17 r89917)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 24.04.4 LTS
#> 
#> Matrix products: default
#> BLAS:   /home/biocbuild/bbs-3.24-bioc/R/lib/libRblas.so 
#> LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.12.0  LAPACK version 3.12.0
#> 
#> locale:
#>  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
#>  [3] LC_TIME=en_GB              LC_COLLATE=C              
#>  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
#>  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
#>  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
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#> 
#> time zone: America/New_York
#> tzcode source: system (glibc)
#> 
#> attached base packages:
#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
#> [8] base     
#> 
#> other attached packages:
#>  [1] chevreulPlot_1.3.0          chevreulProcess_1.3.0      
#>  [3] scater_1.39.4               ggplot2_4.0.2              
#>  [5] scuttle_1.21.6              SingleCellExperiment_1.33.2
#>  [7] SummarizedExperiment_1.41.1 Biobase_2.71.0             
#>  [9] GenomicRanges_1.63.2        Seqinfo_1.1.0              
#> [11] IRanges_2.45.0              S4Vectors_0.49.2           
#> [13] BiocGenerics_0.57.1         generics_0.1.4             
#> [15] MatrixGenerics_1.23.0       matrixStats_1.5.0          
#> [17] BiocStyle_2.39.0           
#> 
#> loaded via a namespace (and not attached):
#>   [1] RColorBrewer_1.1-3        jsonlite_2.0.0           
#>   [3] shape_1.4.6.1             magrittr_2.0.5           
#>   [5] ggbeeswarm_0.7.3          GenomicFeatures_1.63.2   
#>   [7] farver_2.1.2              rmarkdown_2.31           
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#>  [11] BiocIO_1.21.0             vctrs_0.7.3              
#>  [13] memoise_2.0.1             Rsamtools_2.27.2         
#>  [15] DelayedMatrixStats_1.33.0 RCurl_1.98-1.18          
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#>  [53] S7_0.2.1-1                BiocParallel_1.45.0      
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#>  [79] XVector_0.51.0            foreach_1.5.2            
#>  [81] stringr_1.6.0             ggrepel_0.9.8            
#>  [83] pillar_1.11.1             limma_3.67.1             
#>  [85] circlize_0.4.18           dplyr_1.2.1              
#>  [87] lattice_0.22-9            rtracklayer_1.71.3       
#>  [89] bit_4.6.0                 tidyselect_1.2.1         
#>  [91] ComplexHeatmap_2.27.1     locfit_1.5-9.12          
#>  [93] Biostrings_2.79.5         knitr_1.51               
#>  [95] gridExtra_2.3             bookdown_0.46            
#>  [97] ProtGenerics_1.43.0       edgeR_4.9.8              
#>  [99] cmdfun_1.0.2              xfun_0.57                
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#> [133] KEGGREST_1.51.1