\name{bgcorrect} \alias{bgcorrect} \alias{bgcorrect.gc} \alias{bgcorrect.mas4} \alias{bgcorrect.mas5} \alias{bgcorrect.rma} \alias{xpsBgCorrect-methods} \alias{xpsBgCorrect} \title{Background Correction} \description{ Background corrects probe intensities in an object of class \code{\link{DataTreeSet}}. } \usage{ bgcorrect(xps.data, filename = character(0), filedir = getwd(), tmpdir = "", update = FALSE, select = "none", method = character(0), option = character(0), exonlevel = "", params = list(), verbose = TRUE) bgcorrect.gc(xps.data, filename = character(0), filedir = getwd(), tmpdir = "", update = FALSE, select = "antigenomic", exonlevel = "", verbose = TRUE) bgcorrect.mas4(xps.data, filename = character(0), filedir = getwd(), tmpdir = "", update = FALSE, select = "all", exonlevel = "", verbose = TRUE) bgcorrect.mas5(xps.data, filename = character(0), filedir = getwd(), tmpdir = "", update = FALSE, select = "both", exonlevel = "", verbose = TRUE) bgcorrect.rma(xps.data, filename = character(0), filedir = getwd(), tmpdir = "", update = FALSE, select = "none", exonlevel = "", verbose = TRUE) xpsBgCorrect(object, ...) } \arguments{ \item{xps.data}{object of class \code{DataTreeSet}.} \item{filename}{file name of ROOT data file.} \item{filedir}{system directory where ROOT data file should be stored.} \item{tmpdir}{optional temporary directory where temporary ROOT files should be stored.} \item{update}{logical. If \code{TRUE} the existing ROOT data file \code{filename} will be updated.} \item{select}{type of probes to select for background correction.} \item{method}{background method to use.} \item{option}{type of background correction to use.} \item{exonlevel}{exon annotation level determining which probes should be used for summarization; exon/genome arrays only.} \item{params}{vector of parameters for background method.} \item{verbose}{logical, if \code{TRUE} print status information.} \item{object}{object of class \code{DataSet}.} \item{\dots}{the arguments described above.} } \details{ Background corrects probe intensities in an object of class \code{\link{DataTreeSet}}. \code{xpsBgCorrect} is the \code{DataSet} method called by function \code{bgcorrect}, containing the same parameters. } \value{ An \code{\link{DataTreeSet}} } \author{Christian Stratowa} \seealso{\code{\link{express}}} \examples{ ## first, load ROOT scheme file and ROOT data file scheme.test3 <- root.scheme(paste(.path.package("xps"),"schemes/SchemeTest3.root",sep="/")) data.test3 <- root.data(scheme.test3, paste(.path.package("xps"),"rootdata/DataTest3_cel.root",sep="/")) ## MAS4 sector background data.bg.mas4 <- bgcorrect.mas4(data.test3,"tmp_Test3MAS4Bgrd",filedir=getwd(),tmpdir="",verbose=FALSE) ## need to attach background intensities data.bg.mas4 <- attachBgrd(data.bg.mas4) ## get data.frame bg.mas4 <- validBgrd(data.bg.mas4) head(bg.mas4) ## plot images if (interactive()) { image.dev(data.bg.mas4,bg=TRUE,col=rainbow(32)) image(matrix(bg.mas4[,1], ncol=ncols(schemeSet(data.bg.mas4)), nrow=nrows(schemeSet(data.bg.mas4)))) } \dontrun{ ## examples using Affymetrix human tissue dataset (see also xps/examples/script4exon.R) ## example - exon array, e.g. HuEx-1_0-st-v2: scmdir <- "/Volumes/GigaDrive/CRAN/Workspaces/Schemes" datdir <- "/Volumes/GigaDrive/CRAN/Workspaces/ROOTData" scheme.exon <- root.scheme(paste(scmdir,"Scheme_HuEx10stv2r2_na25.root",sep="/")) data.exon <- root.data(scheme.exon, paste(datdir,"HuTissuesExon_cel.root",sep="/")) ## compute rma background workdir <- "/Volumes/GigaDrive/CRAN/Workspaces/Exon/hutissues/exon" data.bg.rma <- bgcorrect(data.exon, "HuExonRMABgrd", filedir=workdir, tmpdir="", method="rma", select="antigenomic", option="pmonly:epanechnikov", params=c(16384), exonlevel="metacore+affx") # or alternatively: data.bg.rma <- bgcorrect.rma(data.exon, "HuExonRMABgrd", filedir=workdir, tmpdir="", select="antigenomic", exonlevel="metacore+affx") } } \keyword{manip}