\name{mvaMicroRna} \alias{mvaMicroRna} \title{MA plot} \description{ For each array, the M value is computed for every spot as the difference between the spot intensity in the array and the median intensity for that feature over the whole set of arrays. Every kind of feature is identified with different color (microRNA genes, positive controls, etc ...) The input must be an RGList object created by the user, in such a way that the RGList$G field contains the expression matrix that we want to use in log2 scale. The gProcessedSignal computed by the Agilent Feature Extaction software normally contains negative values, so a small constant has to be added to the signals before log tranformation. } \usage{ mvaMicroRna(RGlist, maintitle, verbose=FALSE) } \arguments{ \item{RGlist}{An RGlist object. It uses the expression matrix stored in the RGList$G slot. Input expression matrix should be in log2 scale} \item{maintitle}{ character to indicate the title of the graph } \item{verbose}{ logical, if \code{TRUE}it prints details } } \author{ Pedro Lopez-Romero } \examples{ data(dd.micro) op=par(mfrow=c(1,1),ask=TRUE) MMM=dd.micro$Gb ## gProcessedSignal min=min(MMM) ## transforming gProcessedSignal to positive values for(i in 1:dim(MMM)[2]){ ## before log2 transformation MMM[,i]=MMM[,i]+(abs(min)+ 5) } ddaux=dd.micro ddaux$G=log2(MMM) mvaMicroRna(ddaux,maintitle="ProcessedSignal",verbose=FALSE) rm(ddaux) par(op) } \keyword{documentation} \keyword{utilities}