\name{getDecideTests} \alias{getDecideTests} \title{Differential expression analysis an multiplicity of the tests } \description{ It Uses the \code{decideTests} function of the 'limma' package to classify the list of genes as up, down or not significant after correcting by the multiplicity of the tests. } \usage{ getDecideTests(fit2, DEmethod, MTestmethod, PVcut,verbose=FALSE) } \arguments{ \item{fit2}{MArrayLM object } \item{DEmethod}{method for \code{decideTests}, only 'separate' or 'nestedF' are implemented. see \code{decideTests} in limma package. } \item{MTestmethod}{method for multiple test, choices are 'none','BH', 'BY', ... see \code{p.adjust} } \item{PVcut}{p value threshold to declare significant features } \item{verbose}{logical, if \code{TRUE} prints out output} } \value{ A 'TestResults' object of the 'limma' package It prints out the number of UP and DOWN genes for every contrasts according to the p value limit specified } \references{ Smyth, G. K. (2005). Limma: linear models for microarray data. In: 'Bioinformatics and Computational Biology Solutions using R and Bioconductor'. R. Gentleman, V. Carey, S. Dudoit, R. Irizarry, W. Huber (eds), Springer, New York, pages 397--420. } \author{ Pedro Lopez-Romero } \seealso{An overview of miRNA differential expression analysis is given in \code{basicLimma} } \examples{ \dontrun{ DE=getDecideTests(fit2, DEmethod="separate", MTestmethod="BH", PVcut=0.10, verbose=TRUE) } } \keyword{documentation} \keyword{utilities}